Nature Communications (Jun 2020)
Long-term culture of human pancreatic slices as a model to study real-time islet regeneration
- Mirza Muhammad Fahd Qadir,
- Silvia Álvarez-Cubela,
- Jonathan Weitz,
- Julia K. Panzer,
- Dagmar Klein,
- Yaisa Moreno-Hernández,
- Sirlene Cechin,
- Alejandro Tamayo,
- Joana Almaça,
- Helmut Hiller,
- Maria Beery,
- Irina Kusmartseva,
- Mark Atkinson,
- Stephan Speier,
- Camillo Ricordi,
- Alberto Pugliese,
- Alejandro Caicedo,
- Christopher A. Fraker,
- Ricardo Luis Pastori,
- Juan Domínguez-Bendala
Affiliations
- Mirza Muhammad Fahd Qadir
- Diabetes Research Institute, University of Miami Miller School of Medicine
- Silvia Álvarez-Cubela
- Diabetes Research Institute, University of Miami Miller School of Medicine
- Jonathan Weitz
- Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine
- Julia K. Panzer
- Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine
- Dagmar Klein
- Diabetes Research Institute, University of Miami Miller School of Medicine
- Yaisa Moreno-Hernández
- Diabetes Research Institute, University of Miami Miller School of Medicine
- Sirlene Cechin
- Diabetes Research Institute, University of Miami Miller School of Medicine
- Alejandro Tamayo
- Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine
- Joana Almaça
- Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine
- Helmut Hiller
- nPOD Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida
- Maria Beery
- nPOD Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida
- Irina Kusmartseva
- nPOD Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida
- Mark Atkinson
- nPOD Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida
- Stephan Speier
- Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden, Helmholtz Zentrum München
- Camillo Ricordi
- Diabetes Research Institute, University of Miami Miller School of Medicine
- Alberto Pugliese
- Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine
- Alejandro Caicedo
- Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine
- Christopher A. Fraker
- Diabetes Research Institute, University of Miami Miller School of Medicine
- Ricardo Luis Pastori
- Diabetes Research Institute, University of Miami Miller School of Medicine
- Juan Domínguez-Bendala
- Diabetes Research Institute, University of Miami Miller School of Medicine
- DOI
- https://doi.org/10.1038/s41467-020-17040-8
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 15
Abstract
The ability to culture live pancreatic tissue slices for long periods of time would enable longitudinal studies ex vivo. Here the authors culture human and mouse pancreatic slices in a perfluorocarbon-based culture system and show stable endocrine and exocrine function for up to ten days in culture.
