Treatment of Acinetobacter baumannii by Specialized ϕTZ Bacteriophage and Studying of pH Effect on Bacteriophage Stability and Efficacy

Abstract

In general, one of the most important and dangerous pathogens facing human society is the resistance of different types of bacteria to many types of antibiotics. Acinetobacter baumannii is one of the most dangerous pathogens because of its prevalence in the hospital environment, being among the normal flora of bacteria inside the body also they are opportunistic bacteria due to their ability to turn into pathogenic bacteria when there is any weakness in the body’s immunity thus causes many diseases such as pneumonia, inflammation of the central nervous system and many different diseases. Because of the high ability of A. baumannii to acquire genes responsible for resistance to all antibiotics, including carbapenems, from the environment, it has become necessary to discover new methods to limit the spread of this bacteria and treat them. Among these methods is the treatment by bacteriophages then studying the physical properties, and one of the important characteristics that must be studied is the best pH that maintains the stability and effectiveness of the bacteriophage specialized in treating A. baumannii (ϕTZ), which belongs to the family Myoviridae. The pH 6.0 was found to maintain the best activity in the lysis of bacteria and stability of Bacteriophage. As well as, it was able to survive 100%.

Keywords

• Bacteriophages
• Acinetobacter baumannii
• MDR