Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana

Quentin M. Dudley; Seohyun Jo; Delia Ayled Serna Guerrero; Monika Chhetry; Mark A. Smedley; Wendy A. Harwood; Nathaniel H. Sherden; Sarah E. O’Connor; Lorenzo Caputi; Nicola J. Patron

doi:10.1038/s42003-022-03904-w

Communications Biology (Sep 2022)

Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana

Quentin M. Dudley,
Seohyun Jo,
Delia Ayled Serna Guerrero,
Monika Chhetry,
Mark A. Smedley,
Wendy A. Harwood,
Nathaniel H. Sherden,
Sarah E. O’Connor,
Lorenzo Caputi,
Nicola J. Patron

Affiliations

Quentin M. Dudley: Engineering Biology, Earlham Institute, Norwich Research Park
Seohyun Jo: Engineering Biology, Earlham Institute, Norwich Research Park
Delia Ayled Serna Guerrero: Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology
Monika Chhetry: John Innes Centre, Norwich Research Park
Mark A. Smedley: John Innes Centre, Norwich Research Park
Wendy A. Harwood: John Innes Centre, Norwich Research Park
Nathaniel H. Sherden: John Innes Centre, Norwich Research Park
Sarah E. O’Connor: Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology
Lorenzo Caputi: Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology
Nicola J. Patron: Engineering Biology, Earlham Institute, Norwich Research Park

DOI: https://doi.org/10.1038/s42003-022-03904-w
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 12

Abstract

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The biosynthesis of strictosidine, a key intermediate of monoterpene indole alkaloids, was successfully reconstructed in Nicotiana benthamiana, demonstrating the potential of Nicotiana benthamiana as a bioproduction chassis for small molecules.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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