Nature Communications (Aug 2021)
CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
- J. Boutin,
- J. Rosier,
- D. Cappellen,
- F. Prat,
- J. Toutain,
- P. Pennamen,
- J. Bouron,
- C. Rooryck,
- J. P. Merlio,
- I. Lamrissi-Garcia,
- G. Cullot,
- S. Amintas,
- V. Guyonnet-Duperat,
- C. Ged,
- J. M. Blouin,
- E. Richard,
- S. Dabernat,
- F. Moreau-Gaudry,
- A. Bedel
Affiliations
- J. Boutin
- Bordeaux University
- J. Rosier
- Bordeaux University
- D. Cappellen
- Bordeaux University
- F. Prat
- Bordeaux University
- J. Toutain
- Bordeaux University, MRGM INSERM U1211, CHU de Bordeaux, Service de Génétique Médicale
- P. Pennamen
- Bordeaux University, MRGM INSERM U1211, CHU de Bordeaux, Service de Génétique Médicale
- J. Bouron
- Bordeaux University, MRGM INSERM U1211, CHU de Bordeaux, Service de Génétique Médicale
- C. Rooryck
- Bordeaux University
- J. P. Merlio
- Bordeaux University
- I. Lamrissi-Garcia
- Bordeaux University
- G. Cullot
- Bordeaux University
- S. Amintas
- Bordeaux University
- V. Guyonnet-Duperat
- INSERM US 005—CNRS UMS 342-TBM-Core, Bordeaux University
- C. Ged
- Bordeaux University
- J. M. Blouin
- Bordeaux University
- E. Richard
- Bordeaux University
- S. Dabernat
- Bordeaux University
- F. Moreau-Gaudry
- Bordeaux University
- A. Bedel
- Bordeaux University
- DOI
- https://doi.org/10.1038/s41467-021-25190-6
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 12
Abstract
CRISPR-Cas9 generates double-strand breaks, which pose a threat to genome integrity. Here the authors show loss of heterozygosity in edited hematopoietic progenitor cells.
