Frontiers in Plant Science (Jun 2024)

Exploring regulatory network of icariin synthesis in Herba Epimedii through integrated omics analysis

  • Xuedong Zhu,
  • Shiqi Wen,
  • Shiqi Wen,
  • Hameed Gul,
  • Pan Xu,
  • Yang Yang,
  • Yang Yang,
  • Ximei Liao,
  • Ximei Liao,
  • Yunling Ye,
  • Yunling Ye,
  • Zijian Xu,
  • Zijian Xu,
  • Xiaofang Zhang,
  • Lin Wu,
  • Lin Wu,
  • Lin Wu

DOI
https://doi.org/10.3389/fpls.2024.1409601
Journal volume & issue
Vol. 15

Abstract

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Herba Epimedii’s leaves are highly valued in traditional Chinese medicine for their substantial concentration of flavonoids, which play a crucial role in manifesting the plant’s therapeutic properties. This study investigated the metabolomic, transcriptomic and proteomic profiles of leaves from two Herba Epimedii cultivars, Epipremnum sagittatum (J) and Epipremnum pubescens (R), at three different developmental stages. Metabolite identification and analysis revealed a total of 1,412 and 1,421 metabolites with known structures were found. Flavonoids made up of 33%, including 10 significant accumulated icariin analogues. Transcriptomic analysis unveiled totally 41,644 differentially expressed genes (DEGs) containing five encoded genes participated in icariin biosynthesis pathways. Totally, 9,745 differentially expressed proteins (DEPs) were found, including Cluster-47248.2.p1 (UDP-glucuronosy/UDP-glucosyltransferase), Cluster-30441.2.p1 (O-glucosyltransferase), and Cluster-28344.9.p1 (anthocyanidin 3-O-glucoside 2 “-O-glucosyltransferase-like) through proteomics analysis which are involved to icariin biosynthesis. Protein-protein interaction (PPI) assay exhibited, totally 12 proteins showing a strong relationship of false discovery rate (FDR) <0.05 with these three proteins containing 2 leucine-rich repeat receptor kinase-like protein SRF7, and 5 methyl jasmonate esterase 1. Multi-omics connection networks uncovered 237 DEGs and 72 DEPs exhibited significant associations with the 10 icariin analogues. Overall, our integrated omics approach provides comprehensive insights into the regulatory network underlying icariin synthesis in Herba Epimedii, offering valuable resources for further research and development in medicinal plant cultivation and pharmaceutical applications.

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