Scientific Reports (Aug 2024)

A novel lipophilic amiloride derivative efficiently kills chemoresistant breast cancer cells

  • Michelle Hu,
  • Ruiwu Liu,
  • Noemi Castro,
  • Liliana Loza Sanchez,
  • Lapamas Rueankham,
  • Julie A. Learn,
  • Ruiqi Huang,
  • Kit S. Lam,
  • Kermit L. Carraway

DOI
https://doi.org/10.1038/s41598-024-71181-0
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 13

Abstract

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Abstract Derivatives of the potassium-sparing diuretic amiloride are preferentially cytotoxic toward tumor cells relative to normal cells, and have the capacity to target tumor cell populations resistant to currently employed therapeutic agents. However, a major barrier to clinical translation of the amilorides is their modest cytotoxic potency, with estimated IC50 values in the high micromolar range. Here we report the synthesis of ten novel amiloride derivatives and the characterization of their cytotoxic potency toward MCF7 (ER/PR-positive), SKBR3 (HER2-positive) and MDA-MB-231 (triple negative) cell line models of breast cancer. Comparisons of derivative structure with cytotoxic potency toward these cell lines underscore the importance of an intact guanidine group, and uncover a strong link between drug-induced cytotoxicity and drug lipophilicity. We demonstrate that our most potent derivative called LLC1 is preferentially cytotoxic toward mouse mammary tumor over normal epithelial organoids, acts in the single digit micromolar range on breast cancer cell line models representing all major subtypes, acts on cell lines that exhibit both transient and sustained resistance to chemotherapeutic agents, but exhibits limited anti-tumor effects in a mouse model of metastatic breast cancer. Nonetheless, our observations offer a roadmap for the future optimization of amiloride-based compounds with preferential cytotoxicity toward breast tumor cells.