Zhongguo youzhi (Oct 2022)

牡丹籽多肽微胶囊的制备、表征及缓释性能Preparation, characterization and sustained-release performance of peony seed polypeptide microcapsule

  • 钱森和1，李方凯1，屠西猛1，郑鹏1，王若男1，赵世光1,2 QIAN Senhe1, LI Fangkai1, TU Ximeng1, ZHENG Peng1, WANG Ruonan1, ZHAO Shiguang1,2

DOI
https://doi.org/10.19902/j.cnki.zgyz.1003-7969.210489
Journal volume & issue
Vol. 47, no. 10
pp. 33 – 38

Abstract

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在分析牡丹籽多肽抗氧化活性的基础上，采用锐孔凝固浴法制备牡丹籽多肽微胶囊，以提高牡丹籽多肽的稳定性。以包埋率为指标，采用单因素实验和响应面实验对牡丹籽多肽微胶囊制备过程中的CaCl2质量分数、海藻酸钠质量分数、芯壁比和反应温度进行了优化，并对牡丹籽多肽微胶囊进行了表征和缓释性能分析。结果表明:牡丹籽多肽对羟自由基、ABTS+自由基和DPPH自由基具有较好的清除能力；牡丹籽多肽微胶囊的最佳制备工艺条件为CaCl2质量分数2.20%、海藻酸钠质量分数180%、芯壁比1∶ 3、反应温度52 ℃，在此条件下包埋率为83.17%;牡丹籽多肽微胶囊的红外光谱相对于牡丹籽多肽发生了红移，且吸收强度低于牡丹籽多肽；扫描电镜观察表明，牡丹籽多肽微胶囊大小约为07 mm,在低倍镜下观察其表面有凹陷和裂痕,在高倍镜下观察其表面有褶皱现象;牡丹籽多肽微胶囊在人工模拟胃液中可以稳定存在，在人工模拟肠液中可以缓慢释放，3 h时释放率达到了95.43%。 Based on the analysis of antioxidant activity of peony seed polypeptide, the peony seed polypeptide microcapsules were prepared by sharp hole coagulation method to improve the stability of peony seed polypeptide. Taking the embedding rate as the index, the mass fractions of CaCl2 and sodium alginate, core-wall ratio and reaction temperature in the preparation of peony seed polypeptide microcapsules were optimized by single factor experiment and response surface experiment. Meanwhile, the peony seed polypeptide microcapsules were characterized and the sustained-release performance were analyzed.The results showed that the peony seed polypeptide had good scavenging capacity for hydroxyl radical, ABTS+ radical and DPPH radical. The optimal preparation conditions of microcapsules were obtained as follows: CaCl2 mass fraction 220%, sodium alginate mass fraction 1.80%, core-wall ratio 1∶ 3, and reaction temperature 52 ℃. Under these conditions, the embedding rate was 83.17%. The infrared spectrum of peony seed polypeptide microcapsule was red shifted compared with peony seed polypeptide, and the absorption intensity was less than that of peony seed polypeptide. Scanning electron microscopy showed that the size of peony seed polypeptide microcapsule was about 0.7 mm, and there were pits and cracks on its surface under low power microscope and many folds under high power microscope. Peony seed polypeptide microcapsules could exist stably in artificially simulated gastric juice and release slowly in artificially simulated intestinal juice, and the release rate reached 95.43% after 3 h.

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