Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells
Hassan Noorbazargan,
Sobhan Amintehrani,
Aghigh Dolatabadi,
Ainaz Mashayekhi,
Nazanin Khayam,
Pooria Moulavi,
Mohammad Naghizadeh,
Amir Mirzaie,
Fatmeh Mirzaei rad,
Mahsa Kavousi
Affiliations
Hassan Noorbazargan
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences
Sobhan Amintehrani
Department of Biology, East Tehran Branch, Islamic Azad University
Aghigh Dolatabadi
Department of Biology, Tehran North Branch, Islamic Azad University
Ainaz Mashayekhi
Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University
Nazanin Khayam
Department of Biology, East Tehran Branch, Islamic Azad University
Pooria Moulavi
Department of Biology, East Tehran Branch, Islamic Azad University
Mohammad Naghizadeh
Department of Biology, Central Tehran Branch, Islamic Azad University
Amir Mirzaie
Department of Biology, Parand Branch, Islamic Azad University
Fatmeh Mirzaei rad
Department of Biology, Parand Branch, Islamic Azad University
Mahsa Kavousi
Department of Biology, East Tehran Branch, Islamic Azad University
Abstract The current study evaluated the anti-cancer properties of bio-functionalized silver nanoparticles fabricated by Juniperus chinensis leaf extracts. The nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, dynamic light scattering, Zeta potential and X-ray spectroscopy. Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. The size of the spherical nanoparticle was 12.96 nm with negative zeta potential. Up-regulation of caspase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, and ROS production indicated the remarkable apoptotic effect of AgNPs compared to cisplatin. Moreover, down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs. Cell cycle analysis and downregulation of cyclin D1 indicated cancer cell cessation in the G0/G1 phase. Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line.
