Cold-Activated Lipid Dynamics in Adipose Tissue Highlights a Role for Cardiolipin in Thermogenic Metabolism
Matthew D. Lynes,
Farnaz Shamsi,
Elahu Gosney Sustarsic,
Luiz O. Leiria,
Chih-Hao Wang,
Sheng-Chiang Su,
Tian Lian Huang,
Fei Gao,
Niven R. Narain,
Emily Y. Chen,
Aaron M. Cypess,
Tim J. Schulz,
Zachary Gerhart-Hines,
Michael A. Kiebish,
Yu-Hua Tseng
Affiliations
Matthew D. Lynes
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
Farnaz Shamsi
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
Elahu Gosney Sustarsic
The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark
Luiz O. Leiria
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
Chih-Hao Wang
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
Sheng-Chiang Su
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
Tian Lian Huang
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
Fei Gao
BERG, Framingham, MA 01701, USA
Niven R. Narain
BERG, Framingham, MA 01701, USA
Emily Y. Chen
BERG, Framingham, MA 01701, USA
Aaron M. Cypess
NIH, Bethesda, MD 20814, USA
Tim J. Schulz
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA; German Institute of Human Nutrition, Potsdam-Rehbrücke, Germany
Zachary Gerhart-Hines
The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark
Michael A. Kiebish
BERG, Framingham, MA 01701, USA
Yu-Hua Tseng
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA; Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA; Corresponding author
Summary: Thermogenic fat expends energy during cold for temperature homeostasis, and its activity regulates nutrient metabolism and insulin sensitivity. We measured cold-activated lipid landscapes in circulation and in adipose tissue by MS/MSALL shotgun lipidomics. We created an interactive online viewer to visualize the changes of specific lipid species in response to cold. In adipose tissue, among the approximately 1,600 lipid species profiled, we identified the biosynthetic pathway of the mitochondrial phospholipid cardiolipin as coordinately activated in brown and beige fat by cold in wild-type and transgenic mice with enhanced browning of white fat. Together, these data provide a comprehensive lipid bio-signature of thermogenic fat activation in circulation and tissue and suggest pathways regulated by cold exposure. : In this resource, Lynes et al. use a sensitive MS/MSALL technique to profile over 1,000 lipid species in the blood and adipose tissue of humans and mice exposed to cold. Using the online viewer they created to view these data, pathways activated by cold exposure can be identified. Keywords: brown adipose tissue, white adipose tissue, lipidomics, thermogenesis, cardiolipin
