Frontiers in Molecular Neuroscience (Jan 2020)
Differential Expression Profiles and Functional Prediction of tRNA-Derived Small RNAs in Rats After Traumatic Spinal Cord Injury
- Chuan Qin,
- Chuan Qin,
- Chuan Qin,
- Chuan Qin,
- Chuan Qin,
- Hao Feng,
- Hao Feng,
- Hao Feng,
- Hao Feng,
- Hao Feng,
- Chao Zhang,
- Chao Zhang,
- Chao Zhang,
- Chao Zhang,
- Chao Zhang,
- Xin Zhang,
- Xin Zhang,
- Xin Zhang,
- Xin Zhang,
- Xin Zhang,
- Yi Liu,
- Yi Liu,
- Yi Liu,
- Yi Liu,
- Yi Liu,
- De-Gang Yang,
- De-Gang Yang,
- De-Gang Yang,
- De-Gang Yang,
- De-Gang Yang,
- Liang-Jie Du,
- Liang-Jie Du,
- Liang-Jie Du,
- Liang-Jie Du,
- Liang-Jie Du,
- Ying-Chun Sun,
- Ying-Chun Sun,
- Ming-Liang Yang,
- Ming-Liang Yang,
- Ming-Liang Yang,
- Ming-Liang Yang,
- Ming-Liang Yang,
- Feng Gao,
- Feng Gao,
- Feng Gao,
- Feng Gao,
- Feng Gao,
- Jian-Jun Li,
- Jian-Jun Li,
- Jian-Jun Li,
- Jian-Jun Li,
- Jian-Jun Li
Affiliations
- Chuan Qin
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Chuan Qin
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Chuan Qin
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Chuan Qin
- Chinese Institute of Rehabilitation Science, Beijing, China
- Chuan Qin
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Hao Feng
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Hao Feng
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Hao Feng
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Hao Feng
- Chinese Institute of Rehabilitation Science, Beijing, China
- Hao Feng
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Chao Zhang
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Chao Zhang
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Chao Zhang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Chao Zhang
- Chinese Institute of Rehabilitation Science, Beijing, China
- Chao Zhang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Xin Zhang
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Xin Zhang
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Xin Zhang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Xin Zhang
- Chinese Institute of Rehabilitation Science, Beijing, China
- Xin Zhang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Yi Liu
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Yi Liu
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Yi Liu
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Yi Liu
- Chinese Institute of Rehabilitation Science, Beijing, China
- Yi Liu
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- De-Gang Yang
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- De-Gang Yang
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- De-Gang Yang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- De-Gang Yang
- Chinese Institute of Rehabilitation Science, Beijing, China
- De-Gang Yang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Liang-Jie Du
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Liang-Jie Du
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Liang-Jie Du
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Liang-Jie Du
- Chinese Institute of Rehabilitation Science, Beijing, China
- Liang-Jie Du
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Ying-Chun Sun
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Ying-Chun Sun
- TCM Treatment Center, China Rehabilitation Research Center, Beijing, China
- Ming-Liang Yang
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Ming-Liang Yang
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Ming-Liang Yang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Ming-Liang Yang
- Chinese Institute of Rehabilitation Science, Beijing, China
- Ming-Liang Yang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Feng Gao
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Feng Gao
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Feng Gao
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Feng Gao
- Chinese Institute of Rehabilitation Science, Beijing, China
- Feng Gao
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Jian-Jun Li
- School of Rehabilitation Medicine, Capital Medical University, Beijing, China
- Jian-Jun Li
- Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, China
- Jian-Jun Li
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Jian-Jun Li
- Chinese Institute of Rehabilitation Science, Beijing, China
- Jian-Jun Li
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- DOI
- https://doi.org/10.3389/fnmol.2019.00326
- Journal volume & issue
-
Vol. 12
Abstract
Spinal cord injury (SCI) is mostly caused by trauma. As the primary mechanical injury is unavoidable, a focus on the underlying molecular mechanisms of the SCI-induced secondary injury is necessary to develop promising treatments for patients with SCI. Transfer RNA-derived small RNA (tsRNA) is a novel class of short, non-coding RNA, possessing potential regulatory functions in various diseases. However, the functional roles of tsRNAs in traumatic SCI have not been determined yet. We used a combination of sequencing, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), bioinformatics, and luciferase reporter assay to screen the expression profiles and identify the functional roles of tsRNAs after SCI. As a result, 297 differentially expressed tsRNAs were identified in rats’ spinal cord 1 day after contusion. Of those, 155 tsRNAs were significantly differentially expressed: 91 were significantly up-regulated, whereas 64 were significantly down-regulated after SCI (fold change > 1.5; P < 0.05). Bioinformatics analyses revealed candidate tsRNAs (tiRNA-Gly-GCC-001, tRF-Gly-GCC-012, tRF-Gly-GCC-013, and tRF-Gly-GCC-016) that might play regulatory roles through the mitogen-activated protein kinase (MAPK) and neurotrophin signaling pathways by targeting brain-derived neurotrophic factor (BDNF). We validated the candidate tsRNAs and found opposite trends in the expression levels of the tsRNAs and BDNF after SCI. Finally, tiRNA-Gly-GCC-001 was identified to target BDNF using the luciferase reporter assay. In summary, we found an altered tsRNA expression pattern and predicted tiRNA-Gly-GCC-001 might be involved in the MAPK and neurotrophin pathways by targeting the BDNF, thus regulating the post-SCI pathophysiologic processes. This study provides novel insights for future investigations to explore the mechanisms and therapeutic targets for SCI.
Keywords
