Wound Healing Effect of Essential Oil Extracted from <i>Eugenia dysenterica</i> DC (Myrtaceae) Leaves
Sandra Márcia Mazutti da Silva,
Claudio Rodrigues Rezende Costa,
Guilherme Martins Gelfuso,
Eliete Neves Silva Guerra,
Yanna Karla de Medeiros Nóbrega,
Sueli Maria Gomes,
Aline Pic-Taylor,
Yris Maria Fonseca-Bazzo,
Damaris Silveira,
Pérola de Oliveira Magalhães
Affiliations
Sandra Márcia Mazutti da Silva
Natural Products Laboratory, School of Health Sciences, University of Brasília, 70910-900, Campus Universitário Darcy Ribeiro, 70910-900 Brasília-DF, Brazil
Claudio Rodrigues Rezende Costa
Laboratory of Oral Histopathology, Health Sciences Faculty, University of Brasília, 70910-900 Brasília, Brazil
Guilherme Martins Gelfuso
Laboratory of Food, Drugs and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70910-900 Campus Universitario Darcy Ribeiro, Brasília-DF 70910-900, Brazil
Eliete Neves Silva Guerra
Laboratory of Oral Histopathology, Health Sciences Faculty, University of Brasília, 70910-900 Brasília, Brazil
Yanna Karla de Medeiros Nóbrega
Natural Products Laboratory, School of Health Sciences, University of Brasília, 70910-900, Campus Universitário Darcy Ribeiro, 70910-900 Brasília-DF, Brazil
Sueli Maria Gomes
Department of Botany, Institute of Biological Sciences, University of Brasília, Brasília, Campus Universitário Darcy Ribeiro, Brasília-DF 70910-900, Brazil
Aline Pic-Taylor
Laboratory of Embryology and Developmental Biology, Genetics and Morphology Department, Institute of Biological Sciences, University of Brasília, 70910-900, Campus Universitário Darcy Ribeiro, Brasília-DF 70910-900, Brazil
Yris Maria Fonseca-Bazzo
Natural Products Laboratory, School of Health Sciences, University of Brasília, 70910-900, Campus Universitário Darcy Ribeiro, 70910-900 Brasília-DF, Brazil
Damaris Silveira
Natural Products Laboratory, School of Health Sciences, University of Brasília, 70910-900, Campus Universitário Darcy Ribeiro, 70910-900 Brasília-DF, Brazil
Pérola de Oliveira Magalhães
Natural Products Laboratory, School of Health Sciences, University of Brasília, 70910-900, Campus Universitário Darcy Ribeiro, 70910-900 Brasília-DF, Brazil
The use of natural oils in topical pharmaceutical preparations has usually presented safe agents for the improvement of human health. Based on research into the immense potential of wound management and healing, we aimed to validate the use of topical natural products by studying the ability of the essential oil of Eugenia dysenterica DC leaves (oEd) to stimulate in vitro skin cell migration. Skin cytotoxicity was evaluated using a fibroblast cell line (L929) by MTT assay. The oil chemical profile was investigated by GC-MS. Moreover, the inhibition of lipopolysaccharide (LPS) induced nitric oxide (NO) production in the macrophage cell line (RAW 264.7) tested. The Chick Chorioallantoic Membrane (CAM) assay was used to evaluate the angiogenic activity and irritating potential of the oil. The oEd induces skin cell migration in a scratch assay at a concentration of 542.2 µg/mL. α-humulene and β-caryophyllene, the major compounds of this oil, as determined by GC-MS, may partly explain the migration effect. The inhibition of nitric oxide by oEd and α-humulene suggested an anti-inflammatory effect. The CAM assay showed that treatment with oEd ≤ 292 µg/mL did not cause skin injury, and that it can promote angiogenesis in vivo. Hence, these results indicate the feasibility of the essential oil of Eugenia dysenterica DC leaves to developed dermatological products capable of helping the body to repair damaged tissue.
