Frontiers in Plant Science (Jan 2024)
Differential gene expression and potential regulatory network of fatty acid biosynthesis during fruit and leaf development in yellowhorn (Xanthoceras sorbifolium), an oil-producing tree with significant deployment values
- Tian-Le Shi,
- Tian-Le Shi,
- Hai-Yao Ma,
- Hai-Yao Ma,
- Xinrui Wang,
- Xinrui Wang,
- Hui Liu,
- Hui Liu,
- Xue-Mei Yan,
- Xue-Mei Yan,
- Xue-Chan Tian,
- Xue-Chan Tian,
- Zhi-Chao Li,
- Zhi-Chao Li,
- Yu-Tao Bao,
- Yu-Tao Bao,
- Zhao-Yang Chen,
- Zhao-Yang Chen,
- Shi-Wei Zhao,
- Shi-Wei Zhao,
- Qiuhong Xiang,
- Qiuhong Xiang,
- Kai-Hua Jia,
- Shuai Nie,
- Shuai Nie,
- Wenbin Guan,
- Wenbin Guan,
- Jian-Feng Mao,
- Jian-Feng Mao,
- Jian-Feng Mao
Affiliations
- Tian-Le Shi
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Tian-Le Shi
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Hai-Yao Ma
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Hai-Yao Ma
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Xinrui Wang
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Xinrui Wang
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Hui Liu
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Hui Liu
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Xue-Mei Yan
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Xue-Mei Yan
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Xue-Chan Tian
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Xue-Chan Tian
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Zhi-Chao Li
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Zhi-Chao Li
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Yu-Tao Bao
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Yu-Tao Bao
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Zhao-Yang Chen
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Zhao-Yang Chen
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Shi-Wei Zhao
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Shi-Wei Zhao
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Qiuhong Xiang
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Qiuhong Xiang
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Kai-Hua Jia
- Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Ji’nan, China
- Shuai Nie
- Guangdong Academy of Agricultural Sciences & Key Laboratory of Genetics and Breeding of High-Quality Rice in Southern China (Co-construction by Ministry and Province), Rice Research Institute, Guangzhou, China
- Shuai Nie
- Ministry of Agriculture and Rural Affairs & Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Research Institute, Guangzhou, China
- Wenbin Guan
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Wenbin Guan
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Jian-Feng Mao
- State Key Laboratory of Tree Genetics and Breeding, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Jian-Feng Mao
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, School of Ecology and Nature Conservation, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Jian-Feng Mao
- Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, Umeå, Sweden
- DOI
- https://doi.org/10.3389/fpls.2023.1297817
- Journal volume & issue
-
Vol. 14
Abstract
Xanthoceras sorbifolium (yellowhorn) is a woody oil plant with super stress resistance and excellent oil characteristics. The yellowhorn oil can be used as biofuel and edible oil with high nutritional and medicinal value. However, genetic studies on yellowhorn are just in the beginning, and fundamental biological questions regarding its very long-chain fatty acid (VLCFA) biosynthesis pathway remain largely unknown. In this study, we reconstructed the VLCFA biosynthesis pathway and annotated 137 genes encoding relevant enzymes. We identified four oleosin genes that package triacylglycerols (TAGs) and are specifically expressed in fruits, likely playing key roles in yellowhorn oil production. Especially, by examining time-ordered gene co-expression network (TO-GCN) constructed from fruit and leaf developments, we identified key enzymatic genes and potential regulatory transcription factors involved in VLCFA synthesis. In fruits, we further inferred a hierarchical regulatory network with MYB-related (XS03G0296800) and B3 (XS02G0057600) transcription factors as top-tier regulators, providing clues into factors controlling carbon flux into fatty acids. Our results offer new insights into key genes and transcriptional regulators governing fatty acid production in yellowhorn, laying the foundation for efforts to optimize oil content and fatty acid composition. Moreover, the gene expression patterns and putative regulatory relationships identified here will inform metabolic engineering and molecular breeding approaches tailored to meet biofuel and bioproduct demands.
Keywords
- fatty acid biosynthesis
- time-ordered gene co-expression network (TO-GCN)
- oleosins
- fruit and leaf development
- yellowhorn
