Microbial diversity, transformation and toxicity of azo dye biodegradation using thermo-alkaliphilic microbial consortia

Abstract

In this research, the transformation and toxicity of Reactive Red 141 and 239 biodegraded under anaerobic-aerobic conditions as well as metagenomic analysis of Reactive Red 239 degrading microbial consortia collected from Shala Hot spring were investigated. Toxicity of dyes before treatment and after treatment on three plants, fish and microorganisms were done. A halotolerant and thermo-alkaliphilic bacterial consortia decolorizing azo dyes (>98% RR 141 and > 96% RR 239 in 7 h) under optimum conditions of salt concentration (0.5%), temperature (55 °C) and pH (9), were used. Toxicity effect of untreated dyes and treated dyes in Tomato > Beetroot > Cabbage plants, while the effect was Leuconostoc mesenteroides > Lactobacillus plantarum > Escherichia coli in microorganisms. Among fishes, the toxicity effect was highest in Oreochromis niloticus followed by Cyprinus carpio and Clarias gariepinus. The three most dominant phyla that could be in charge of decolorizing RR 239 under anaerobic-aerobic systems were Bacteroidota (22.6–29.0%), Proteobacteria (13.5–29.0%), and Chloroflexi (8.8–23.5%). At class level microbial community structure determination, Bacteroidia (18.9–27.2%), Gammaproteobacteria (11.0–15.8%), Alphaproteobacteria (2.5–5.0%) and Anaerolineae (17.0–21.9%) were dominant classes. The transformation of RR 141 and RR 239 into amine compounds were proposed via high performance liquid chromatography-mass spectroscopy (HPLC/MS) and fourier transform infrared spectroscopy (FT-IR). Overall, dye containing wastewaters treated under anaerobic-aerobic systems using thermo-alkaliphilic microbial consortia were found to be safe to agricultural (fishes and vegetables) purposes.

Keywords

• Consortia
• FT-IR
• Metagenomic analysis
• Shala hot spring
• Thermo-alkaliphiles
• Toxicity