Burkholderia cepacia isolated from varieties of Yam with antimicrobial activity against Colletotrichum gloeosporioides

Abstract

The present study had as objective in vitro to evaluate the antifungal activity of type compound antibiotic produced by isolated endophytes bacteria from varieties of yam against the mycelial growth of the fungus C. gloesporioides. Samples of Yam plants were collected randomly in the form of zig-zag in the Department of Sucre. The collected samples were disinfected surface; subsequently is led to out the isolation, counting and separation of morphotypes of endophytes bacteria through technique of serial over surface of the agar medium. Each to isolated morphotype was used to evaluate in vitro inhibitory activity of cells of endophytes bacteria on the growth of the fungus C. gloesporioides. The morphotype of bacteria with greater inhibitory activity were selected to obtain metabolites antibiotic type in two sources of carbon (glucose and mannitol) in three concentrations (0,5; 1,0 and 1,5%). The morphotype with greater inhibitory activity was 3AT1, the results of the in vitro test showed greater activity when the composite type antibiotic was obtained in the middle with a concentration of 1,5% glucose. Identification with kit API20E results confirm with a 99.9% of identity with the kind of bacterium Burkholderia cepacia, becoming this compound as a potential biological against the micelal growth of the fungus C. gloesporioides, causing the disease known as yam anthracnose in the Department of Sucre.

Keywords

• Microorganismos endófito
• hongos fitopatógenos
• metabolito
• inhibición.