An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking
Ramachandran Chelliah,
Kandasamy Saravanakumar,
Eric Banan-Mwine Daliri,
Joong-Hark Kim,
Jung-Kun Lee,
Hyeon-yeong Jo,
Inamul Hasan Madar,
Se-Hun Kim,
Sudha Rani Ramakrishnan,
Momna Rubab,
Kaliyan Barathikannan,
Fred Kwame Ofosu,
Hwang Subin,
Park Eun-ji,
Fazle Elahi,
Myeong-Hyeon Wang,
Deog-Hwan Oh
Affiliations
Ramachandran Chelliah
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Kandasamy Saravanakumar
Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Eric Banan-Mwine Daliri
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Joong-Hark Kim
Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea; Erom, Co., Ltd., Chuncheon, Gangwon-do 24427, Korea
Jung-Kun Lee
Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea; Erom, Co., Ltd., Chuncheon, Gangwon-do 24427, Korea
Hyeon-yeong Jo
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Inamul Hasan Madar
Department of Biochemistry, School of Life Science, Bharathidasan, University, Thiruchirappalli, Tamilnadu, India
Se-Hun Kim
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Sudha Rani Ramakrishnan
School of Food Science and Biotechnology, Kyungpook National University, Daegu, South Korea
Momna Rubab
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Kaliyan Barathikannan
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Fred Kwame Ofosu
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Hwang Subin
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Park Eun-ji
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Fazle Elahi
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Myeong-Hyeon Wang
Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea
Deog-Hwan Oh
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea; Corresponding author.
Most of the probiotics Bacterial cells, express native antibacterial genes, resulting in the production of, antimicrobial peptides, which have various applications in biotechnology and drug development. But the identification of antibacterial peptide, structural characterization of antimicrobial peptide and prediction on mode of action. Regardless of the significance of protein manufacturing, three individual factors are required for the production method: gene expression, stabilization and specific peptide purification. Our protocol describes a straightforward technique of detecting and characterizing particular extracellular peptides and enhancing the antimicrobial peptide expression we optimized using low molecular weight peptides. This protocol can be used to improve peptide detection and expression. The following are the benefits of this method, (DOI – https://doi.org/10.1016/j.ijbiomac.2019.10.196 [1]).The data briefly describe a simple method in detection identification, characterization of antimicrobial extracellular peptide, predicating the mode of action of peptide in targeting pathogens (In-silico method), brief method on profiling of antimicrobial peptide and its mode of action [1]. Further the protocol can be used to enhance the specific peptide expressions, detection of peptides. The advantages of this technique are presented below: • Characterization protocol of specific antimicrobial peptide • The folded antimicrobial peptide expression were less expressed or non-expressed peptides. • Besides being low cost, less time-consuming, easy to handle, universal and fast to execute, the suggested technique can be used for multiple proteins expressed in probiotics (Lactobacillus species) expression system.
