Determination of the Infection Dynamics of <i>Escherichia coli</i> O157:H7 by Bacteriophage ΦV10
Michael F. Oats,
Claudia P. Coronel-Aguilera,
Bruce M. Applegate,
Laszlo N. Csonka,
Arun K. Bhunia,
Andrew G. Gehring,
George C. Paoli
Affiliations
Michael F. Oats
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
Claudia P. Coronel-Aguilera
Department of Food Science, Purdue University, West Lafayette, IN 47907, USA
Bruce M. Applegate
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
Laszlo N. Csonka
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
Arun K. Bhunia
Department of Food Science, Purdue University, West Lafayette, IN 47907, USA
Andrew G. Gehring
Characterization and Interventions for Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, PA 19038, USA
George C. Paoli
Characterization and Interventions for Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, PA 19038, USA
ΦV10 is an Escherichia coli O157:H7-specific bacteriophage that has been used to develop luminescent reporter assays for the detection of this important foodborne pathogen. Previous work demonstrated the specificity of ΦV10 for infection of E.coli O157:H7 through interaction with the O157 antigen. In addition, modification of the lipopolysaccharide (LPS) via O-acetylation prevents ΦV10 infection in an E. coli O157:H7 expressing a phage-encoded O-acetylase gene. Through assays for phage binding, plaque formation, and lysogeny using non-O157:H7 and O157: non-H7 strains, as well as complementation of an O157:H− strain, it is demonstrated in this study that both the somatic O157 antigen and flagellar H7 antigen are required for productive infection of E. coli O157:H7 by ΦV10. Together, the results indicate that the O157 antigen is required for phage binding and that the H7 antigen is necessary to complete the infection process.
