The Protective Effects of Long-Term Probiotic Application on Experimental Sepsis-dependent Inflammation Process

Abstract

Objective:Probiotics are defined as live microorganisms that provide beneficial effects on the host when applied in appropriate amounts. The immun-modulatory effects of some probiotics are one of these. We aimed to investigate the effects of probiotics on some inflammatory cytokine levels in sepsis.Methods:The mixture including 12 different live-probiotic bacteria was used in this study. Group 1 and 2 were evaluated as control and sepsis groups. Sepsis was developed in groups 2, 3, 4, 7 and 8 using cecal ligation and puncture. The probiotic mixture was given to rats in groups 3 and 4 for 21 days before sepsis, groups 7 and 8 a single dose after sepsis at 1010 and 1011 doses, respectively. Rats in groups 5 and 6, the same doses of probiotics were administered without sepsis. Interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-10 and transforming growth factor (TGF)-β levels were measured by ELISA method.Results:There was a serious increase on the level of TNF-α and IL-1β levels in sepsis group, and a serious decrease in 21 days of protective treatment groups. In accordance with IL-10 and TGF-β levels, although the cytokine levels of 21 days protective treatment groups were not reached in control group, a serious increase was observed compared to the sepsis group.Conclusion:According to our findings, probiotics can be used for supportive purposes in addition to classical medical treatment of sepsis. However, we believe that more studies should be performed in order to standardize the effects of probiotic and determine its effects with different experimental groups of volunteers as well as experimental animal studies.

Keywords

• Inflammation
• sepsis
• cytokine
• probiotic