Preparation of gelatin/Ag NPs under ultrasound condition: A potent and green bio-nanocomposite for the treatment of pleomorphic hepatocellular carcinoma, morris hepatoma, and novikoff hepatoma

Zukuan Chang; Bikash Karmakar; Huilin Lu; Xuelei Lou; Shadi Salem Alkhayyat; Gomaa Mostafa-Hedeab; Gaber El-Saber Batiha; Attalla F. El-kott; Fahmy G. Elsaid; Mohamed A. Al-Kahtani; Mutasem Z. Bani-Fwaz

Arabian Journal of Chemistry (Jun 2022)

Preparation of gelatin/Ag NPs under ultrasound condition: A potent and green bio-nanocomposite for the treatment of pleomorphic hepatocellular carcinoma, morris hepatoma, and novikoff hepatoma

Zukuan Chang,
Bikash Karmakar,
Huilin Lu,
Xuelei Lou,
Shadi Salem Alkhayyat,
Gomaa Mostafa-Hedeab,
Gaber El-Saber Batiha,
Attalla F. El-kott,
Fahmy G. Elsaid,
Mohamed A. Al-Kahtani,
Mutasem Z. Bani-Fwaz

Affiliations

Zukuan Chang: Department of Intervention Therapy, Xinxiang Central Hospital, The Fourth Clinical College of Xinxiang Medical University, Xinxiang City, Henan Province, 453000, China
Bikash Karmakar: Department of Chemistry, Gobardanga Hindu College, 24 Parganas (North), India; Corresponding author.
Huilin Lu: Department of Intervention Therapy, Xinxiang Central Hospital, The Fourth Clinical College of Xinxiang Medical University, Xinxiang City, Henan Province, 453000, China
Xuelei Lou: Department of Intervention Therapy, Xinxiang Central Hospital, The Fourth Clinical College of Xinxiang Medical University, Xinxiang City, Henan Province, 453000, China
Shadi Salem Alkhayyat: Department of Internal Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Gomaa Mostafa-Hedeab: Department of Pharmacology & Health Research Unit – Medical College, Jouf University, Jouf, Saudai Arabia; Department of Pharmacology, Faculty of Medicine, Beni-Suef University, Egypt
Gaber El-Saber Batiha: Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt
Attalla F. El-kott: Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia; Department of Zoology, Faculty of Science, Damanhour University, Damanhour, Egypt
Fahmy G. Elsaid: Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia; Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt
Mohamed A. Al-Kahtani: Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia
Mutasem Z. Bani-Fwaz: Department of Chemistry, College of Science, King Khalid University, P. O. Box 9004, Abha 61413, Saudi Arabia

Journal volume & issue: Vol. 15, no. 6
p. 103858

Abstract

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Regarding applicative, facile, green chemical research, a bio-inspired approach is being reported for the sonochemical synthesis of Ag nanoparticles by gelatin as a natural reducing/stabilizing and solid support agent without using any toxic and harmful reagent. The biosynthesized Ag NPs@gelatin bionanocomposite were characterized by advanced physicochemical techniques like ultraviolet–visible (UV–Vis), Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), High-Resolution Transmission Electron Microscopy (HR-TEM), Energy Dispersive X-ray spectroscopy (EDX), and X-ray Diffraction (XRD) study. It has been established that gelatin-stabilized silver nanoparticles have a spherical shape with a mean diameter from ∼5–10 nm. To survey the antioxidant potentials of Ag NPs@gelatin, one of the common antioxidant techniques i.e., DPPH was used. The results of DPPH test proved excellent antioxidant properties of Ag NPs@gelatin in comparison to the positive control used i.e., butylated hydroxytoluene. The IC50 of Ag NPs@gelatin was 250 µg/mL in the antioxidant test. Determination of anti-liver cancer effects of Ag NPs@gelatin was carried out by the MTT assay and against pleomorphic hepatocellular carcinoma (SNU-387), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cell lines. The results of MTT assay confirmed removing SNU-387, McA-RH7777, and N1-S1 Fudr cell lines after treating with low concentrations of Ag NPs@gelatin. The IC50 of the Ag NPs@gelatin was 192, 144, and 228 µg/mL against SNU-387, McA-RH7777, and N1-S1 Fudr cell lines, respectively. The best result of anti-human liver cancer effects of Ag NPs@gelatin against the above cell lines was seen in the case of the N1-S1 Fudr cell line. According to the above results, significant anti-liver cancer effects of Ag NPs@gelatin is confirmed.

Published in Arabian Journal of Chemistry

ISSN: 1878-5352 (Print)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry
Website: http://www.journals.elsevier.com/arabian-journal-of-chemistry/

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