Tissue Factor Expression of Endothelial Cell in Response to Atherosclerotic Risk Factors

Weerasak  Wongtiraporn; Pusadee  Luenee; Nisarat  Opartkiattikul; Wanida  Wongtiraporn; Wanida  Wongtiraporn; Somsak  Laiwejpithaya; Suthi  Sangkarat; Sujera  Laiwejpithaya

Siriraj Medical Journal (Mar 2020)

Tissue Factor Expression of Endothelial Cell in Response to Atherosclerotic Risk Factors

Weerasak Wongtiraporn,
Pusadee Luenee,
Nisarat Opartkiattikul,
Wanida Wongtiraporn,
Wanida Wongtiraporn,
Somsak Laiwejpithaya,
Suthi Sangkarat,
Sujera Laiwejpithaya

Affiliations

Weerasak Wongtiraporn: Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Pusadee Luenee: Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Nisarat Opartkiattikul: Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Wanida Wongtiraporn: Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Wanida Wongtiraporn: Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Somsak Laiwejpithaya: Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Suthi Sangkarat: Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Sujera Laiwejpithaya: Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700

Journal volume & issue: Vol. 63, no. 1

Abstract

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Objective: To study the effect of serum from patients with atherosclerotic risk factors on the synthesis of endothelial tissue factor. Methods: Serum from 30 diabetic patients, 30 hyperlipemic patients, 30 smokers and 30 normal serum were incubated with cultured endothelial cells from a human umbilical vein. The tissue factor of endothelial cells was measured using the assay that was developed in house after 24 hours incubation time. Results: Smokersั serum can significantly cause the increase in endothelial tissue factor. The mean level of tissue factor induced by smokersั serum is 1.12 microunits/cell whereas the mean level of tissue factor induced by diabetic serum, hyperlipemic serum and normal serum is 0.4, 0.48 and 0.2 microunit/cell, respectively. Conclusion: Smoking may increase the risk of thrombosis by increasing the tissue factor production of endothelial cells.

Endothelial cell, tissue factor; risk factors

Published in Siriraj Medical Journal

ISSN: 2228-8082 (Online)
Publisher: Faculty of Medicine Siriraj Hospital
Country of publisher: Thailand
LCC subjects: Medicine
Website: https://he02.tci-thaijo.org/index.php/sirirajmedj/about

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