miR-19a-3p promotes the growth of hepatocellular carcinoma by regulating p53/SOX4
Hang Zhang,
Jiajun Zhu,
Jingjun Zhang,
Ying Liu,
Baicheng Zhao,
Xiaoyi Yang,
Wenhan Zhou,
Bozhou Chen,
Shuangshuang Zhang,
Ruotong Huang,
Shuying Chen
Affiliations
Hang Zhang
Department of Laboratory Medicine, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China; Medical College, Fudan University, 130 Dongan Road, Shanghai, 200032, China
Jiajun Zhu
Department of Laboratory Medicine, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China; Medical College, Fudan University, 130 Dongan Road, Shanghai, 200032, China
Jingjun Zhang
Department of Rehabilitation Medicine, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, China
Ying Liu
Medical College, Fudan University, 130 Dongan Road, Shanghai, 200032, China
Baicheng Zhao
Medical College, Fudan University, 130 Dongan Road, Shanghai, 200032, China
Xiaoyi Yang
Department of Laboratory Medicine, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China
Wenhan Zhou
Medical College, Fudan University, 130 Dongan Road, Shanghai, 200032, China
Bozhou Chen
Department of Laboratory Medicine, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China
Shuangshuang Zhang
Department of Laboratory Medicine, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China
Ruotong Huang
Department of Laboratory Medicine, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China; Medical College, Fudan University, 130 Dongan Road, Shanghai, 200032, China
Shuying Chen
Department of Laboratory Medicine, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China; Corresponding author.
Objective: This study aims to investigate the potential functions of miR-19a-3p in HCC. Method: We collected serum samples to analyze miR-19a-3p expression. We utilized CCK8 and Transwell assays to access miR-19a-3p′s influence on HCC cells malignancy. We used dual-luciferase reporter and western blotting to validate the impact of p53/miR-19 on miR-19/SOX4. Results: The results demonstrated that miR-19a-3p was highly expressed in pre-operative serum samples and HCC cells, which can promote cell proliferation, migration and invasion in HCC under in vitro conditions. Additionally, there was a p53 binding site on the upstream of miR-19a-3p, which was inhibited by p53. SOX4 was the direct gene targeted by miR-19a-3p. The imbalance of p53-miR-19-SOX4 loop was one reason for the progress of HCC. Conclusion: Our findings validate the mechanisms of miR-19a-3p and highlight its potential as a therapeutic target in HCC.
