Generation and characterization of human-derived iPSC lines from one pair of dizygotic twins discordant for schizophrenia

Peiyan Ni; Huiyao Wang; Jia Cai; Junzhe Ran; Youhui Jiang; Liansheng Zhao; Jinxue Wei; Rongjun Ni; Yingcheng Wang; Xiaohong Ma; Qiang Wang; Wanjun Guo; Tao Li

Stem Cell Research (Apr 2022)

Generation and characterization of human-derived iPSC lines from one pair of dizygotic twins discordant for schizophrenia

Peiyan Ni,
Huiyao Wang,
Jia Cai,
Junzhe Ran,
Youhui Jiang,
Liansheng Zhao,
Jinxue Wei,
Rongjun Ni,
Yingcheng Wang,
Xiaohong Ma,
Qiang Wang,
Wanjun Guo,
Tao Li

Affiliations

Peiyan Ni: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Huiyao Wang: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Jia Cai: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Junzhe Ran: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Youhui Jiang: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Liansheng Zhao: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Jinxue Wei: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Rongjun Ni: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Yingcheng Wang: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Xiaohong Ma: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Qiang Wang: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Wanjun Guo: The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China
Tao Li: Department of Neurobiology, Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou 310013, Zhejiang, China; NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, Zhejiang, China; Corresponding author at: Affiliated Mental Health Centre & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou 310013, Zhejiang, China.

Journal volume & issue: Vol. 60
p. 102710

Abstract

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Schizophrenia (SCZ) is a debilitating neurodevelopmental disorder with a high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by a pair of dizygotic twins. The female was clinically diagnosed as SCZ by Diagnostic and Statistical Manual of Mental Disorders IV (DSM-IV) criteria, and her unaffected male sibling was healthy control. Induced pluripotent stem cells (iPSCs) were established using Episomal vectors carrying reprograming factors OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT. These lines with normal karyotype highly expressed pluripotency markers and are capable to differentiate into derivatives of three germ layers. Both lines are negative of mycoplasma.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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