The effect of biopolymer stabilisation on biostimulated or bioaugmented mine residue for potential technosol production

Abstract

Abstract Mine waste can be transformed into technosol as an ecological strategy. Despite its importance to soil functions, biological activity is often overlooked. Biopolymers can serve as innovative tools for bioremediation, facilitating chemical reactions and creating networks to encapsulate contaminants. This work aims to assess the use of bioleached and stabilised residues from a tungsten mine for technosol production. The first objective was to evaluate mine tailings for their bioleaching potential by biostimulation or bioaugmentation with strain Diaphorobacter polyhydroxybutyrativorans B2A2W2. The second was to evaluate the effect of Portland cement or biopolymers such as Carboxymethyl Cellulose (CMC) or Xanthan Gum (XG) on the stabilisation of bioleached residues. The impact of biopolymers on residues’ characteristics, such as metal leaching, number of cultivable microorganisms, compression strength and ecotoxicity was evaluated using flow systems. Over time, bioleached metallic elements decreased, except for iron (Fe). Biostimulated and stabilised residues exhibited similar trends; both CMC and cement showed low leaching rates and viable microorganisms in the same order (106 CFU × ml−1). However, bioaugmented residue stabilised with XG showed 106 CFU × ml−1 viable microorganisms and increased 2.2-fold Fe leaching than BA_Control. CMC addition to bioaugmented residue reduced 5.9-fold Fe leaching and increased 100-fold viable microorganisms. By utilising both biological and engineering approaches to characterise the technosol, this study contributes to advancing knowledge of technosol production. The residues biostimulated and stabilised with CMC produced a material useful for bio-applications, with low toxicity and metal leaching, useful for bio-applications. XG was the best stabiliser for geotechnical engineering applications, with improved compression strength. In conclusion, the study demonstrates the usefulness of biopolymer treatment for residues and emphasises the importance of selecting the appropriate biopolymer for the intended function of technosols.

Keywords

• Bioleaching
• Biopolymers
• Metal recovery
• Waste treatment
• Sustainability