THE EFFECT OF PREDNISONE ON SERTOLI CELL OF CONTRALATERAL TESTICULAR TORSION

Abstract

Objective: To evaluate the effect of prednisone in unilateral testicular torsion on Sertoli cell quality of contralateral testis. Material & Method: Thirty Wistar rats were divided into three groups i.e. group A (sham procedure), group B (unilateral torsion + orchiectomy after 6 hours), and group C (unilateral torsion + orchiectomy after 24 hours). Group B and C were further divided into subgroup with and without Prednisone administration. Prednisone was given orally once a day an hour after torsion, continued till one month later. Orchiectomy of contralateral testis was performed one month later. Those testes were examined by a pathologist. Results: In group A, no abnormality on Sertoli cell quality was found. There is significant difference among groups in Sertoli cell quality (p = 0,01). Ischemic time is associated with Sertoli cell quality (2 rats with severe damage in group C and none in group B). In group B and C, prednisone administration inhibited Sertoli cell damage. Prednisone administration in 6 hours group gave better results than 24 hours group (3 rats vs 1 rat with good Sertoli cell quality). Conclusion: Sertoli cell in contralateral testis is significantly affected by unilateral testicular torsion. Prednisone inhibit Sertoli cells damage. Ischemic time affected Sertoli cell quality of contralateral testis and respond to prednisone. Keywords: Prednisone, unilateral testicular torsion, sertoli cell.