Purification and characterization of cutinase from Botrytis cinerea and effect on blueberry cuticle

Meng Liu; Zihao Zong; Xiangjun Fang; Ruiling Liu; Honglei Mu; Hangjun Chen; Ben Niu; Haiyan Gao

Journal of Agriculture and Food Research (Jun 2023)

Purification and characterization of cutinase from Botrytis cinerea and effect on blueberry cuticle

Meng Liu,
Zihao Zong,
Xiangjun Fang,
Ruiling Liu,
Honglei Mu,
Hangjun Chen,
Ben Niu,
Haiyan Gao

Affiliations

Meng Liu: Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China; Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, China; Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, China; Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China; College of Food Science and Engineering, Ocean University of China, China
Zihao Zong: Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China; Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, China; Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, China; Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
Xiangjun Fang: Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China; Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, China; Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, China; Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China; Corresponding author. Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China.
Ruiling Liu: Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China; Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, China; Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, China; Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
Honglei Mu: Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China; Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, China; Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, China; Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
Hangjun Chen: Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China; Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, China; Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, China; Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
Ben Niu: Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China; Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, China; Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, China; Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China; Corresponding author. Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China.
Haiyan Gao: Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China; Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, China; Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, China; Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China; Corresponding author. Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, People's Republic of China.

Journal volume & issue: Vol. 12
p. 100599

Abstract

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An extracellular cutinase in Botrytis cinerea was isolated and its biochemical properties and effects on the penetration of pathogens were evaluated. This cutinase had a specific activity of 6.03 U/μg of protein after 22.33-fold purification, and yielded a recovery of 50.5%. The optimum pH and temperature for the enzymatic activity of cutinase were 9 and 45 °C, respectively. The cutinase was stable between 30 and 80 °C and pH 4.0–10.0. Fe3+ and Cu2+ strongly increased cutinase activity while Mn2+ completely inhibited it. The enzyme could maintain its activity in the presence of methanol, ethanol and isopropanol, but was significantly inhibited in acetonitrile, n-hexane and acetone. Kinetic parameters KM, Vmax and kcat were 0.21 mM, 303 μmol•min−1•mg−1 protein and 303.03 s−1 respectively. The purified cutinase could also destroy blueberry wax crystal and decrease the cuticle, indicating a positive role on the penetration of pathogens for blueberry fruits. This study provides useful information for fruit postharvest preservation.

Published in Journal of Agriculture and Food Research

ISSN: 2666-1543 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General); Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-agriculture-and-food-research

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