Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology

Abstract

Highlights ・MIHS-SAST screening generates mAbs that target protein structures. ・One-fourth of the mAbs recognized stereospecific epitopes of the protein. ・Two fluorometric parameters aided in selecting antibodies with strong binding constants.

Keywords

• Monoclonal antibody
• Flow cytometer
• Conformation-specific antibody
• ELISA
• Hybridoma technology