Virology Journal (Jul 2012)

Evaluating viral interference between Influenza virus and Newcastle disease virus using real-time reverse transcription–polymerase chain reaction in chicken eggs

  • Ge Shengqiang,
  • Zheng Dongxia,
  • Zhao Yunling,
  • Liu Hualei,
  • Liu Wenbo,
  • Sun Qing,
  • Li Jinming,
  • Yu Songmei,
  • Zuo Yuanyuan,
  • Han Xiuju,
  • Li Lin,
  • Lv Yan,
  • Wang Yingli,
  • Liu Xiufan,
  • Wang Zhiliang

DOI
https://doi.org/10.1186/1743-422X-9-128
Journal volume & issue
Vol. 9, no. 1
p. 128

Abstract

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Abstract Background Simultaneous and sequential allantoic cavity inoculations of Specific-pathogen-free (SPF) chicken eggs with Influenza virus (AIV) and Newcastle disease virus (NDV) demonstrated that the interaction of AIV and NDV during co-infection was variable. Our research revisited the replication interference potential of AIV and NDV using real-time reverse transcription–polymerase chain reaction (real-time RT-PCR) for AIV and NDV to specifically detect the viral genomes in mixed infections. Results Data from this survey showed that when different doses of NDV (Lasota or F48E8) and AIV (F98 or H5N1) were simultaneously inoculated into embryonating chicken eggs (ECE), interference with the growth of NDV occurred, while interference with the growth of AIV did not occur. When equal amount of the two viruses were sequentially employed, the degree of interference was dependent upon the time of superinfection and the virulence of virus. Conclusion AIV have a negative impact on NDV growth if they are inoculated simultaneously or sequentially and that the degree of interference depended upon the quantity and relative virulence of the virus strains used; however, interference with AIV was not observed. Only if NDV were inoculated at an earlier time will NDV able to interfere with the growth of AIV.

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