Stem Cell Research (Aug 2022)
In vitro modeling of amyotrophic lateral sclerosis with induced pluripotent stem cell technology-derived cell line ORIONi002-A
- Jan Strnadel,
- Hugo M. Dumortier,
- Dominika Hajduchova,
- Romana Zahumenska,
- Vladimir Nosal,
- Marek Smolar,
- Juraj Marcinek,
- Michal Kalman,
- Sandra Mersakova,
- Dusan Brany,
- Stefan Juhas,
- Jana Juhasova,
- Hana Studenovska,
- Barbora Mitruskova,
- Stanislava Suroviakova,
- Slavomira Novakova,
- Henrieta Skovierova,
- Egon Kurca,
- Renata Pecova,
- Lukas Plank,
- Erika Halasova
Affiliations
- Jan Strnadel
- Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia; Corresponding author.
- Hugo M. Dumortier
- Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia
- Dominika Hajduchova
- Department of Pathological Physiology, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia
- Romana Zahumenska
- Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia
- Vladimir Nosal
- Clinic of Neurology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia
- Marek Smolar
- Clinic of Surgery and Transplant Center, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia
- Juraj Marcinek
- Department of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia
- Michal Kalman
- Department of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia
- Sandra Mersakova
- Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia
- Dusan Brany
- Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia
- Stefan Juhas
- PIGMOD Centre, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., Liběchov, Czech Republic
- Jana Juhasova
- PIGMOD Centre, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., Liběchov, Czech Republic
- Hana Studenovska
- Department of Biomaterials and Bioanalogous Systems Institute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, Czech Republic
- Barbora Mitruskova
- Department of Clinical Genetics, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia
- Stanislava Suroviakova
- Clinic of Children and Adolescents, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia
- Slavomira Novakova
- Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia
- Henrieta Skovierova
- Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia
- Egon Kurca
- Clinic of Neurology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia
- Renata Pecova
- Department of Pathological Physiology, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia
- Lukas Plank
- Department of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, University Hospital, Martin, Slovakia
- Erika Halasova
- Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin (JFM CU), Comenius University in Bratislava, Slovakia
- Journal volume & issue
-
Vol. 63
p. 102870
Abstract
We present here a new iPS cell line for modeling sporadic form of ALS. Cell line was generated by reprogramming skin fibroblasts isolated with explant culture technology from skin biopsy, donated by ALS patient. For reprogramming, polycistronic self-replicating RNA vector was used and derived iPS cells were characterized by immunocytochemistry and FACS (pluripotent factors expression), karyotyping, STR fingerprinting analysis and in vitro differentiation assay. New cell line showed normal (46, XY) karyotype and differentiated in vitro into cells from three germ layers. STR analysis proved the origin and originality of the cell line.
