Frontiers in Nutrition (Sep 2022)
Effects of molecular weight on intestinal anti-inflammatory activities of β-D-glucan from Ganoderma lucidum
- Yanfang Liu,
- Yanfang Liu,
- Yanfang Liu,
- Qingjiu Tang,
- Qingjiu Tang,
- Qingjiu Tang,
- Jie Feng,
- Jie Feng,
- Jie Feng,
- Jing Liu,
- Jing Liu,
- Jing Liu,
- Chuanhong Tang,
- Chuanhong Tang,
- Chuanhong Tang,
- Mengqiu Yan,
- Mengqiu Yan,
- Mengqiu Yan,
- Shuai Zhou,
- Shuai Zhou,
- Shuai Zhou,
- Liping Liu,
- Liping Liu,
- Liping Liu,
- Jing Zhou,
- Jing Zhou,
- Jing Zhou,
- Jing Zhou,
- Jingsong Zhang,
- Jingsong Zhang,
- Jingsong Zhang
Affiliations
- Yanfang Liu
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Yanfang Liu
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Yanfang Liu
- National Engineering Research Center of Edible Fungi, Shanghai, China
- Qingjiu Tang
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Qingjiu Tang
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Qingjiu Tang
- National Engineering Research Center of Edible Fungi, Shanghai, China
- Jie Feng
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Jie Feng
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Jie Feng
- National Engineering Research Center of Edible Fungi, Shanghai, China
- Jing Liu
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Jing Liu
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Jing Liu
- National Engineering Research Center of Edible Fungi, Shanghai, China
- Chuanhong Tang
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Chuanhong Tang
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Chuanhong Tang
- National Engineering Research Center of Edible Fungi, Shanghai, China
- Mengqiu Yan
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Mengqiu Yan
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Mengqiu Yan
- National Engineering Research Center of Edible Fungi, Shanghai, China
- Shuai Zhou
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Shuai Zhou
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Shuai Zhou
- National Engineering Research Center of Edible Fungi, Shanghai, China
- Liping Liu
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Liping Liu
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Liping Liu
- National Engineering Research Center of Edible Fungi, Shanghai, China
- Jing Zhou
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Jing Zhou
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Jing Zhou
- National Engineering Research Center of Edible Fungi, Shanghai, China
- Jing Zhou
- Shanghai Baixin Bio-Tech Co., Ltd., Shanghai, China
- Jingsong Zhang
- Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Ministry of Agriculture, Shanghai, China
- Jingsong Zhang
- Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, China
- Jingsong Zhang
- National Engineering Research Center of Edible Fungi, Shanghai, China
- DOI
- https://doi.org/10.3389/fnut.2022.1028727
- Journal volume & issue
-
Vol. 9
Abstract
To investigate the influence of molecular weight (Mw) on the anti-inflammatory activity of β-D-glucan from Ganoderma lucidum, ultrasonic irradiation was applied to treat the β-D-glucan (GLP, 2.42 × 106 g/mol) solution to obtain two degraded fractions with molecular weight of 6.53 × 105 g/mol (GLPC) and 3.49 × 104 g/mol (GLPN). Structural analysis proved that the degraded fractions possessed similar repeated units with the original β-D-glucan. The in vitro anti-inflammatory activity studies showed that all fractions could significantly inhibit LPS-induced expression of cytokines including TNF-α, IL-8, MIF and MCP-1 in Caco-2 cells at certain concentrations. Moreover, GLPC and GLPN exhibited better anti-inflammatory activity than GLPC. The intestinal anti-inflammatory activity evaluated by dextran sulfate sodium (DSS)—induced colitis mice model showed that intragastric administration of GLPN (lower Mw fraction) could significantly recover inflamed tissues of mice. Compared with GLP and GLPC, GLPN exhibited stronger ability to inhibit the secretion of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6). The results revealed that Mw of β-D-glucan influenced its anti-inflammatory activity and decreasing of Mw would improve the activity, which provided evidence for the potential use of β-D-glucan from G. lucidum as anti-colitis ingredients.
Keywords
- Ganoderma lucidum
- β-(1 → 3
- 1 → 6)-D-glucan
- molecular weight
- inflamed Caco-2 cells
- DSS-induced colitis
