Molecular Characterization, Pathogenicity and Biological Characterization of Colletotrichum Species Associated with Anthracnose of Camellia yuhsienensis Hu in China

Xinggang Chen; Lingyu Jiang; Anhua Bao; Changlin Liu; Junang Liu; Guoying Zhou

doi:10.3390/f12121712

Forests (Dec 2021)

Molecular Characterization, Pathogenicity and Biological Characterization of Colletotrichum Species Associated with Anthracnose of Camellia yuhsienensis Hu in China

Xinggang Chen,
Lingyu Jiang,
Anhua Bao,
Changlin Liu,
Junang Liu,
Guoying Zhou

Affiliations

Xinggang Chen: Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Changsha 410004, China
Lingyu Jiang: Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Changsha 410004, China
Anhua Bao: Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Changsha 410004, China
Changlin Liu: Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Changsha 410004, China
Junang Liu: Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Changsha 410004, China
Guoying Zhou: Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Changsha 410004, China

DOI: https://doi.org/10.3390/f12121712
Journal volume & issue: Vol. 12, no. 12
p. 1712

Abstract

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Camellia yuhsienensis Hu, a species of tea oil tree with resistance to anthracnose, is widely used to breed disease-resistant Camellia varieties. In 2019, anthracnose symptoms were observed on Ca. yuhsienensis for the first time. However, the species and biological characteristics of Colletotrichum spp. isolated from Ca. yuhsienensis (YX-Colletotrichum spp.) have not been elucidated. In this study, five isolates (YX2-5-2, 2YX-3-1, 2YX-5-1, 2YX-8-1-1 and 2YX-8-1-2), which were consistent with the morphological characteristics of Colletotrichum spp., were obtained from Ca. yuhsienensis. A phylogenetic analysis demonstrated that YX2-5-2, 2YX-3-1 and 2YX-8-1-2 belonged to first clade along with Colletotrichum fructicola. 2YX-8-1-1 belonged to the second clade along with Colletotrichum siamense. 2YX-5-1 belonged to the third clade with Colletotrichum camelliae. Pathogenicity tests revealed that the pathogenicity of YX-Colletotrichum spp. was stronger than that of Colletotrichum spp. isolated from Camellia oleifera (GD-Colletotrichum spp.). Biological characteristics illustrated that the mycelial growth of YX-Co. camelliae (2YX-5-1) was slower than that of GD-Co. camelliae when the temperature exceeded 20 °C. In addition, in the presence of ions, the mycelial growth of YX-Co. fructicola (YX2-5-2) and YX-Co. siamense (2YX-8-1-1) was also slower than that of GD-Co. fructicola and GD-Co. siamense. Furthermore, the ability of YX-Colletotrichum spp. to utilize lactose and mannitol was weaker than that of GD-Colletotrichum spp., while the ability to utilize NH4+ was generally stronger than that of GD-Colletotrichum spp. This is the first report of anthracnose of Ca. yuhsienensis induced by Co. fructicola, Co. siamense and Co. camelliae in China. These results will provide theoretical guidance for the study of the pathogenesis and control of anthracnose on Ca. yuhsienensis.

Published in Forests

ISSN: 1999-4907 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany: Plant ecology
Website: http://www.mdpi.com/journal/forests/

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