Frontiers in Cellular and Infection Microbiology (Dec 2022)
An algal lectin griffithsin inhibits Hantaan virus infection in vitro and in vivo
- Yajing Zhao,
- Yajing Zhao,
- Ningbo Zhao,
- Ningbo Zhao,
- Yanxing Cai,
- Yanxing Cai,
- Hui Zhang,
- Jia Li,
- Jiaqi Liu,
- Chuantao Ye,
- Yuan Wang,
- Yamei Dang,
- Wanying Li,
- Wanying Li,
- He Liu,
- Lianqing Zhang,
- Yuexiang Li,
- Liang Zhang,
- Linfeng Cheng,
- Yangchao Dong,
- Zhikai Xu,
- Yingfeng Lei,
- Lu Lu,
- Yingjuan Wang,
- Wei Ye,
- Fanglin Zhang
Affiliations
- Yajing Zhao
- College of Life Sciences, Northwest University, Xi’an, Shaanxi, China
- Yajing Zhao
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Ningbo Zhao
- College of Life Sciences, Northwest University, Xi’an, Shaanxi, China
- Ningbo Zhao
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Yanxing Cai
- Guiyang Maternal and Child Health Care Hospital, Guiyang, Guizhou, China
- Yanxing Cai
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences and BSL-3 Facility, Fudan University, Shanghai, China
- Hui Zhang
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Jia Li
- Department of Neurology, Xi’an International Medical Center Hospital, Xi’an, Shaanxi, China
- Jiaqi Liu
- College of Life Sciences, Northwest University, Xi’an, Shaanxi, China
- Chuantao Ye
- Department of Infectious Diseases, Tangdu Hospital, Airforce Medical University, Xi’an, Shaanxi, China
- Yuan Wang
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Yamei Dang
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Wanying Li
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Wanying Li
- Department of Pathogenic Biology, School of Preclinical Medicine, Yan’an University, Yan’an, Shaanxi, China
- He Liu
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Lianqing Zhang
- College of Life Sciences, Northwest University, Xi’an, Shaanxi, China
- Yuexiang Li
- College of Life Sciences, Northwest University, Xi’an, Shaanxi, China
- Liang Zhang
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Linfeng Cheng
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Yangchao Dong
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Zhikai Xu
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Yingfeng Lei
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Lu Lu
- Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences and BSL-3 Facility, Fudan University, Shanghai, China
- Yingjuan Wang
- College of Life Sciences, Northwest University, Xi’an, Shaanxi, China
- Wei Ye
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- Fanglin Zhang
- Department of Microbiology, School of Preclinical Medicine, Airforce Medical University, Xi’an, Shaanxi, China
- DOI
- https://doi.org/10.3389/fcimb.2022.881083
- Journal volume & issue
-
Vol. 12
Abstract
Hantaan virus (HTNV) is the etiological pathogen of hemorrhagic fever with renal syndrome in East Asia. There are currently no effective therapeutics approved for HTNV and other hantavirus infections. We found that griffithsin (GRFT), an algae-derived lectin with broad-spectrum antiviral activity against various enveloped viruses, can inhibit the growth and spread of HTNV. In vitro experiments using recombinant vesicular stomatitis virus (rVSV) with HTNV glycoproteins as a model revealed that the GRFT inhibited the entry of rVSV-HTNV-G into host cells. In addition, we demonstrated that GRFT prevented authentic HTNV infection in vitro by binding to the viral N-glycans. In vivo experiments showed that GRFT partially protected the suckling mice from death induced by intracranial exposure to HTNV. These results demonstrated that GRFT can be a promising agent for inhibiting HTNV infection.
Keywords
- griffithsin (GRFT)
- hantavirus
- Hantaan virus
- hemorrhagic fever with renal syndrome (HFRS)
- lectin
- antivirals
