The HuMet Repository: Watching human metabolism at work
Patrick Weinisch,
Johannes Raffler,
Werner Römisch-Margl,
Matthias Arnold,
Robert P. Mohney,
Manuela J. Rist,
Cornelia Prehn,
Thomas Skurk,
Hans Hauner,
Hannelore Daniel,
Karsten Suhre,
Gabi Kastenmüller
Affiliations
Patrick Weinisch
Institute of Computational Biology, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany
Johannes Raffler
Institute of Computational Biology, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany; Digital Medicine, University Hospital of Augsburg, Augsburg, Germany
Werner Römisch-Margl
Institute of Computational Biology, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany
Matthias Arnold
Institute of Computational Biology, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany; Department of Psychiatry and Behavioral Sciences, Duke University, Durham, NC, USA
Robert P. Mohney
Metabolon, Inc., Durham, NC, USA
Manuela J. Rist
Department of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Karlsruhe, Germany
Cornelia Prehn
Metabolomics and Proteomics Core, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany
Thomas Skurk
ZIEL Institute for Food and Health, Core Facility Human Studies, Technical University of Munich, Freising, Germany; Else Kröner Fresenius Center for Nutritional Medicine, School of Life Sciences, Technical University of Munich, Freising, Germany
Hans Hauner
Else Kröner Fresenius Center for Nutritional Medicine, School of Life Sciences, Technical University of Munich, Freising, Germany; Institute for Nutritional Medicine, School of Medicine and Health, Technical University of Munich, Munich, Germany
Hannelore Daniel
School of Life Sciences, Technical University of Munich, Freising, Germany
Karsten Suhre
Department of Biophysics and Physiology, Weill Cornell Medicine - Qatar, Doha, Qatar
Gabi Kastenmüller
Institute of Computational Biology, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany; Corresponding author
Summary: Metabolism oscillates between catabolic and anabolic states depending on food intake, exercise, or stresses that change a multitude of metabolic pathways simultaneously. We present the HuMet Repository for exploring dynamic metabolic responses to oral glucose/lipid loads, mixed meals, 36-h fasting, exercise, and cold stress in healthy subjects. Metabolomics data from blood, urine, and breath of 15 young, healthy men at up to 56 time points are integrated and embedded within an interactive web application, enabling researchers with and without computational expertise to search, visualize, analyze, and contextualize the dynamic metabolite profiles of 2,656 metabolites acquired on multiple platforms. With examples, we demonstrate the utility of the resource for research into the dynamics of human metabolism, highlighting differences and similarities in systemic metabolic responses across challenges and the complementarity of metabolomics platforms. The repository, providing a reference for healthy metabolite changes to six standardized physiological challenges, is freely accessible through a web portal.
