Frontiers in Immunology (Jul 2022)

Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients

  • Mengyao Wang,
  • Peng Gao,
  • Laifeng Ren,
  • Jingjing Duan,
  • Silu Yang,
  • Haina Wang,
  • Hongxia Wang,
  • Junning Sun,
  • Xiaoyan Gao,
  • Bo Li,
  • Shuaicheng Li,
  • Wen Su

DOI
https://doi.org/10.3389/fimmu.2022.848113
Journal volume & issue
Vol. 13

Abstract

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Cancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determining region 3 (CDR3) of the TCR-beta chain in peripheral blood samples from 68 gastric cancer patients and 49 healthy controls. We found that most hyper-expanded CDR3 are individual-specific, and the gene usage of TRBV3-1 is more frequent in the tumor group regardless of tumor stage than in the healthy control group. We identified 374 hyper-expanded tumor-specific CDR3, which may play a vital role in anti-tumor immune responses. The patients with stage IV gastric cancer have higher EBV-specific CDR3 abundance than the control. In conclusion, analysis of the peripheral blood TCR repertoires may provide the biomarker for gastric cancer prognosis and guide future immunotherapy.

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