Isolation and Characterization of Microsatellite Markers in Brown Planthopper (<em>Nilaparvata </em><em>l</em><em>ugens</em> Stål)

Abstract

Brown planthopper (<em>Nilaparvata lugens</em> Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from <em>N. lugens</em> genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics.

Keywords

• &lt;em&gt;Nilaparvata lugens&lt;/em&gt;
• microsatellites
• polymorphism
• genetic diversity