Genetic Characterization of a blaVIM–24-Carrying IncP-7β Plasmid p1160-VIM and a blaVIM–4-Harboring Integrative and Conjugative Element Tn6413 From Clinical Pseudomonas aeruginosa

Lijun Zeng; Lijun Zeng; Zhe Zhan; Zhe Zhan; Lingfei Hu; Xiaoyuan Jiang; Yanjun Zhang; Jiao Feng; Bo Gao; Yuee Zhao; Wenhui Yang; Huiying Yang; Zhe Yin; Dongsheng Zhou

doi:10.3389/fmicb.2019.00213

Frontiers in Microbiology (Feb 2019)

Genetic Characterization of a blaVIM–24-Carrying IncP-7β Plasmid p1160-VIM and a blaVIM–4-Harboring Integrative and Conjugative Element Tn6413 From Clinical Pseudomonas aeruginosa

Lijun Zeng,
Lijun Zeng,
Zhe Zhan,
Zhe Zhan,
Lingfei Hu,
Xiaoyuan Jiang,
Yanjun Zhang,
Jiao Feng,
Bo Gao,
Yuee Zhao,
Wenhui Yang,
Huiying Yang,
Zhe Yin,
Dongsheng Zhou

Affiliations

Lijun Zeng: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Lijun Zeng: Department of Clinical Laboratory, The 307th Hospital of the Chinese People’s Liberation Army, Beijing, China
Zhe Zhan: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Zhe Zhan: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Lingfei Hu: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Xiaoyuan Jiang: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Yanjun Zhang: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Jiao Feng: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Bo Gao: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Yuee Zhao: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Wenhui Yang: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Huiying Yang: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Zhe Yin: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Dongsheng Zhou: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China

DOI: https://doi.org/10.3389/fmicb.2019.00213
Journal volume & issue: Vol. 10

Abstract

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This study presents three novel integrons In1394, In1395, and In1443, three novel unit transposons Tn6392, Tn6393, and Tn6403, one novel conjugative element (ICE) Tn6413, and the first sequenced IncP-7 resistance plasmid p1160-VIM from clinical Pseudomonas aeruginosa. Detailed sequence comparison of p1160-VIM (carrying Tn6392 and Tn6393) and Tn6413 (carrying Tn6403) with related elements were performed. Tn6392, Tn6393, and Tn6403 were generated from integration of In1394 (carrying blaVIM–24), In1395 and In1443 (carrying blaVIM–4) into prototype Tn3-family unit transposons Tn5563, Tn1403, and Tn6346, respectively. To the best of our knowledge, this is the first report of a blaVIM–24-carrying P. aeruginosa isolate.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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