Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelata

Zhiheng Qiu; Zhiheng Qiu; Shuang Wang; Shuang Wang; Jiazhi Zhao; Jiazhi Zhao; Lingxiu Cui; Lingxiu Cui; Xinyi Wang; Xinyi Wang; Nuo Cai; Nuo Cai; Hongpeng Li; Hongpeng Li; Shuhua Ren; Shuhua Ren; Tianlai Li; Tianlai Li; Lili Shu; Lili Shu

doi:10.3389/fmicb.2023.1276457

Frontiers in Microbiology (Sep 2023)

Synthesis and structural characteristics analysis of melanin pigments induced by blue light in Morchella sextelata

Zhiheng Qiu,
Zhiheng Qiu,
Shuang Wang,
Shuang Wang,
Jiazhi Zhao,
Jiazhi Zhao,
Lingxiu Cui,
Lingxiu Cui,
Xinyi Wang,
Xinyi Wang,
Nuo Cai,
Nuo Cai,
Hongpeng Li,
Hongpeng Li,
Shuhua Ren,
Shuhua Ren,
Tianlai Li,
Tianlai Li,
Lili Shu,
Lili Shu

Affiliations

Zhiheng Qiu: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Zhiheng Qiu: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China
Shuang Wang: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Shuang Wang: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China
Jiazhi Zhao: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Jiazhi Zhao: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China
Lingxiu Cui: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Lingxiu Cui: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China
Xinyi Wang: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Xinyi Wang: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China
Nuo Cai: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Nuo Cai: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China
Hongpeng Li: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Hongpeng Li: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China
Shuhua Ren: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Shuhua Ren: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China
Tianlai Li: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Tianlai Li: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China
Lili Shu: Modern Protected Horticulture Engineering & Technology Center, College of Horticulture, Shenyang Agricultural University, Shenyang, China
Lili Shu: Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, China

DOI: https://doi.org/10.3389/fmicb.2023.1276457
Journal volume & issue: Vol. 14

Abstract

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Morchella sextelata, a highly sought-after edible mushroom worldwide, is evaluated based on its cap color as an essential commercial property indicator. In the present study, the effects of blue light on cap pigmentation in M. sextelata, as well as the synthesis and structural characteristics of melanin pigments within the cap were examined. The results showed that an increase in the proportion of blue light within the lighting environment promoted melanin synthesis and melanization of the cap. Transmission and scanning electron microscopy revealed the localization of melanin within the mycelium and its ultrastructural characteristics. The UV–visible analysis demonstrated that melanin exhibited a maximum absorption peak at 220 nm and possessed high alkaline solubility as well as acid precipitability. The structural characteristics of melanin were analyzed using FTIR, NMR, HPLC, and elemental analysis, which confirmed the presence of eumelanin, pheomelanin, and allomelanin in both brown and black caps. Furthermore, blue light can stimulate the synthesis of both eumelanin and pheomelanin. The obtained results can serve as the foundation for comprehending the mechanism by which light regulates color formation in mushrooms.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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