Identification of carriers of the wheat genotypes stem rust (Puccinia graminis f.sp. tritici) resistance genes

Abstract

Stem rust in recent years has acquired an epiphytotic character, causing significant economic damage for wheat production in some parts of Kazakhstan. On the basis of a race composition study of the stem rust populations collected in 2016-2017 in South-Eastern and northern regions of Kazakhstan were identified. The race differentiation of stem rust using a tester set of 6 Sr genes differentiator lines was carried out. In different conditions of Kazakhstan, the Sr22 and Sr25/Lr19 gene sources are effective. As a result of PCR analysis, the Sr22 gene was found in 5 lines of 6 samples taken for the study: Line C-19SB, Lutescens 7-04-4, Lutescens 220-03-45, GA961662-1-7/TAM107, Line D 25 77. Sr25/Lr19 is a carrier of sources: Lutescens 220-03-45, Advance, Line D 25 77. The Sr22 and Sr25/Lr19 gene was Lutescens 220-03-45 and Line D 25 77. These sources are recommended for inclusion in the breeding process for developing stem rust resistant varieties in the region.