Protective Effect of Minocycline on Bax and Bcl-2 Gene Expression, Histological Damages and Oxidative Stress Induced by Ovarian Torsion in Adult Rats

Abstract

Background: Minocycline is a widely used bacteriostatic antibiotic with various functions. The aim of this study wasto investigate impact of apoptotic genes in ovary of the torsion/detorsion treated rat model by minocycline.Materials and Methods: This experimental study was performed in 32 female Wistar rats classified in four groups,including: i. sham, ii. TD: torsion/detorsion group received normal saline, iii. TDM: torsion/detorsion group treatedwith 40 mg/kg Minocycline, and iv. MC: healthy group received 40 mg/kg Minocycline. After treatment period (7days), histoplogical parameters, oxidative stress markers and hormone profile of serum as well as the expression ofBax and Bcl-2 genes were measured in the ovary of rats.Results: Levels of superoxide dismutase (SOD), glutathione peroxidase (GPX) and estrogen were decreased in theTD group and significantly increased in the treated groups (P=0.001). Levels of malondialdehyde (MDA) and testosteronewere increased in the TD group and decreased in the treated groups (P=0.001). Expression level of Bax waselevated in the TD group, while it was attenuated in the treated groups (P=0.001). Expression level of Bcl-2 wassignificantly increased in treated groups (P=0.001).Conclusion: Minocycline can repair oxidative damage in ovarian tissue and regulate apoptotic-related gene expressions.

Keywords

• ganirelix acetate
• gonadotrophin-releasing hormone antagonist
• in vitro fertilization
• letrozole
• ovarian hyperstimulation syndrome