Optimized protocol for immunophenotyping of melanoma and tumor-bearing skin from mouse

Sai Sakktee Krisna; Christophe Goncalves; Natascha Gagnon; Fan Huang; Dany Plourde; Wilson H. Miller, Jr.; Jörg H. Fritz; Sonia V. del Rincon

STAR Protocols (Sep 2021)

Optimized protocol for immunophenotyping of melanoma and tumor-bearing skin from mouse

Sai Sakktee Krisna,
Christophe Goncalves,
Natascha Gagnon,
Fan Huang,
Dany Plourde,
Wilson H. Miller, Jr.,
Jörg H. Fritz,
Sonia V. del Rincon

Affiliations

Sai Sakktee Krisna: Department of Physiology, Faculty of Medicine, McGill University, Montreal, QC H3G 1Y6, Canada; McGill University Research Centre on Complex Traits, Faculty of Medicine, Bellini Pavilion, McGill University, Montreal, QC H3G 0B1, Canada; Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC H3T 1E2, Canada
Christophe Goncalves: Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC H3T 1E2, Canada
Natascha Gagnon: Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC H3T 1E2, Canada
Fan Huang: Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC H3T 1E2, Canada; Divsion of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada
Dany Plourde: Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC H3T 1E2, Canada
Wilson H. Miller, Jr.: Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC H3T 1E2, Canada; Divsion of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada; Department of Oncology, Faculty of Medicine, McGill University, Montreal, QC H4A 3T2, Canada
Jörg H. Fritz: Department of Physiology, Faculty of Medicine, McGill University, Montreal, QC H3G 1Y6, Canada; McGill University Research Centre on Complex Traits, Faculty of Medicine, Bellini Pavilion, McGill University, Montreal, QC H3G 0B1, Canada; Department of Microbiology and Immunology, Faculty of Medicine, McGill University, Montreal, QC H3A 2B4, Canada; Corresponding author
Sonia V. del Rincon: Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC H3T 1E2, Canada; Divsion of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada; Department of Oncology, Faculty of Medicine, McGill University, Montreal, QC H4A 3T2, Canada; Corresponding author

Journal volume & issue: Vol. 2, no. 3
p. 100627

Abstract

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Summary: While isolating immune cells from spleens and lungs is routinely achieved using flow cytometry, it is challenging to isolate viable immune cells from skin. Here, we describe a step-by-step protocol for skin digestion using a murine melanoma model, which is amenable for detection of low abundant immune cell populations including group 2 innate lymphoid cells.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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