Intermittent hypoxia hypobaric exposure minimized oxidative stress and antioxidants in brain cells of Sprague Dawleymice

Abstract

AbstrakLatar belakang: Hipoksia hypobaric meningkatkan produksi radikal bebas, terutama spesies oksigen reaktif (ROS). Peningkatan ROS akan menyebabkan stres oksidatif bila tidak disertai dengan peningkatan enzim antioksidan. Kondisi ini dapat dikurangi dengan hipoksia hipobarik intermiten (HHI). Tujuan penelitian ini mengidentifikasi frekuensi IHH yang dapat meminimalkan efek hipoksia hipobarik terhadap stres oksidatif dan aktivitas antioksidan spesifik pada tikus Sprague Dawley.Metode: Penelitian eksperimental pada bulan Februari-April 2010, Subjek terdiri dari satu kelompok kontrol dan empat kelompok paparan pada mencit jantan Sprague Dawley. Setiap kelompok terdiri dari 5 tikus. Kelompok kontrol tidak terpapar IHH. Kelompok terpapar (dengan selang waktu satu minggu) terpapar sekali, dua kali, tiga kali, atau empat kali IHH. Semua kelompok paparan dipaparkan hipobarik setara dengan ketinggian: 35.000 ft (1 menit), 25.000 ft (5 menit), dan 18.000 ft (25 menit). Jaringan otak diperiksa untuk 8-OHdG dan SOD.Hasil:Setelah tiga paparan IHH tingkat 8-OHdG sudah kembali ke nilai kontrol (P = 0,843). Tingkat SOD meningkat secara progresif pada dua, tiga, dan empat kali paparan IHH. Bahkan setelah paparan kedua, tingkat SOD sudah sama dengan nilai kontrol, 0,231 ± 0,042 (P = 0,191).Kesimpulan: Tiga kali IHH sudah dapat meminimalkan pengaruh hipoksia hipobarik terhadap stres oksidatif dan aktivitas spesifik antioksidan pada tikus Sprague Dawley.Kata kunci: hipoksia hipobarik intermiten, stres oksidatif, antioksidanAbstractBackground: Hypoxia hypobaric increase the production of free radicals, especially reactive oxygen species (ROS). The increase in ROS would cause oxidative stress when not accompanied by an increase in antioxidant enzymes. This condition may minimize by intermittent hypobaric hypoxia (IHH). This study aimed to identify the number of IHH which may minimize the effect of hypoxia hypobaric on oxidative stress and the specific activity of antioxidants in Sprague Dawley male mice.Methods: The experimental study was in February-April 2010 consisted of one control group and four exposed groups of male mice Sprague Dawley. Each groups consisted of 5 mice. The control group did not have IHH. The exposed groups (with an interval of one week) had once, twice, three, or four times IHH using a chamber flight. All exposed groups were treated hypobaric equivalent to: 35,000 ft altitude (1 minutes), 25,000 ft (5 minutes), and 18,000 ft (25 minutes). All of their brains had 8-OHdG and SOD measured.Results: The 8-OHdG level among three time IHH exposures had already returned to the control value (P = 0.843). The SOD level increased progressively among two, three, and four times IHH. However after the second exposure, it was found that the SOD level was similar to the control value, 0.231 ± 0.042 (P = 0.191).Conclusion: In conclusion, three times of IHH may improve the effect of hypoxia hypobaric on oxidative stress and specific activity of antioxidants in Sprague Dawley male mice. The SOD level was increased at an earlier exposure, which was after one IHH exposure.Keywords: intermittent hypoxia hypobaric, oxidative stress, antioxidants

Keywords

• intermittent hypoxia hypobaric, oxidative stress, antioxidants