A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae, Optimized by Response Surface Methodology

Energies. 2014;7(5):3383-3399 DOI 10.3390/en7053383

 

Journal Homepage

Journal Title: Energies

ISSN: 1996-1073 (Print)

Publisher: MDPI AG

LCC Subject Category: Technology

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

Carlos Luna (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)
Cristobal Verdugo (Laboratorio de Estudios Cristalográficos, IACT, CSIC-University of Granada, Avenida de las Palmeras 4, 18100 Armilla, Granada, Spain)
Enrique D. Sancho (Department of Microbiology, University of Córdoba, Campus de Rabanales, Ed. Severo Ochoa, 14014 Córdoba, Spain)
Diego Luna (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)
Juan Calero (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)
Alejandro Posadillo (Seneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, Spain)
Felipa M. Bautista (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)
Antonio A. Romero (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 11 weeks

 

Abstract | Full Text

A new biodiesel-like biofuel is obtained by the enzymatic ethanolysis reaction of sunflower oil with ethanol, in free solvent media, by using BIOLIPASE-R, a multipurpose alimentary additive from Biocon®-Spain that is a low cost lipase from a strain of Rhizopus oryzae. This biofuel is composed by two parts of fatty acid ethyl esters (FAEE) and one of monoglyceride (MG), which in this form integrates glycerol, through the application of the 1,3-selective lipases. Thus, this process minimizes waste generation and maximizes the efficiency of the process because no residual glycerol is produced. Response surface methodology (RSM) is employed to evaluate the main reaction parameters (reaction temperature, oil/ethanol ratio and pH) on the sunflower oil conversion. Water content and amount of lipase were also previously investigated. Regarding the results, we found that it operates optimally with a water content of the reaction medium of 0.15%, 0.05%–0.1% lipase by weight relative to the weight of oil used, 20 °C, volume ratio (mL/mL) oil/ethanol 12/3.5 and pH 12 (by addition of 50 µL of 10 N NaOH solution). These results have proven a very good efficiency of the biocatalyst in the studied selective process.