Expansion of multipotent stem cells from the adult human brain.

PLoS ONE. 2013;8(8):e71334 DOI 10.1371/journal.pone.0071334


Journal Homepage

Journal Title: PLoS ONE

ISSN: 1932-6203 (Online)

Publisher: Public Library of Science (PLoS)

LCC Subject Category: Medicine | Science

Country of publisher: United States

Language of fulltext: English

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Wayne Murrell
Emily Palmero
John Bianco
Biljana Stangeland
Mrinal Joel
Linda Paulson
Bernd Thiede
Zanina Grieg
Ingunn Ramsnes
Håvard K Skjellegrind
Ståle Nygård
Petter Brandal
Cecilie Sandberg
Einar Vik-Mo
Sheryl Palmero
Iver A Langmoen


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Time From Submission to Publication: 24 weeks


Abstract | Full Text

The discovery of stem cells in the adult human brain has revealed new possible scenarios for treatment of the sick or injured brain. Both clinical use of and preclinical research on human adult neural stem cells have, however, been seriously hampered by the fact that it has been impossible to passage these cells more than a very few times and with little expansion of cell numbers. Having explored a number of alternative culturing conditions we here present an efficient method for the establishment and propagation of human brain stem cells from whatever brain tissue samples we have tried. We describe virtually unlimited expansion of an authentic stem cell phenotype. Pluripotency proteins Sox2 and Oct4 are expressed without artificial induction. For the first time multipotency of adult human brain-derived stem cells is demonstrated beyond tissue boundaries. We characterize these cells in detail in vitro including microarray and proteomic approaches. Whilst clarification of these cells' behavior is ongoing, results so far portend well for the future repair of tissues by transplantation of an adult patient's own-derived stem cells.