Daily Eicosapentaenoic Acid Infusion in IUGR Fetal Lambs Reduced Systemic Inflammation, Increased Muscle ADRβ2 Content, and Improved Myoblast Function and Muscle Growth

Haley N. Beer; Taylor A. Lacey; Rachel L. Gibbs; Micah S. Most; Zena M. Hicks; Pablo C. Grijalva; Eileen S. Marks-Nelson; Ty B. Schmidt; Jessica L. Petersen; Dustin T. Yates

doi:10.3390/metabo14060340

Metabolites (Jun 2024)

Daily Eicosapentaenoic Acid Infusion in IUGR Fetal Lambs Reduced Systemic Inflammation, Increased Muscle ADRβ2 Content, and Improved Myoblast Function and Muscle Growth

Haley N. Beer,
Taylor A. Lacey,
Rachel L. Gibbs,
Micah S. Most,
Zena M. Hicks,
Pablo C. Grijalva,
Eileen S. Marks-Nelson,
Ty B. Schmidt,
Jessica L. Petersen,
Dustin T. Yates

Affiliations

Haley N. Beer: Stress Physiology Laboratory, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Taylor A. Lacey: Stress Physiology Laboratory, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Rachel L. Gibbs: Stress Physiology Laboratory, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Micah S. Most: Stress Physiology Laboratory, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Zena M. Hicks: Stress Physiology Laboratory, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Pablo C. Grijalva: Stress Physiology Laboratory, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Eileen S. Marks-Nelson: Stress Physiology Laboratory, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Ty B. Schmidt: Meat Science and Muscle Biology, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Jessica L. Petersen: Animal Breeding and Genetics, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
Dustin T. Yates: Stress Physiology Laboratory, Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA

DOI: https://doi.org/10.3390/metabo14060340
Journal volume & issue: Vol. 14, no. 6
p. 340

Abstract

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Intrauterine growth-restricted (IUGR) fetuses exhibit systemic inflammation that contributes to programmed deficits in myoblast function and muscle growth. Thus, we sought to determine if targeting fetal inflammation improves muscle growth outcomes. Heat stress-induced IUGR fetal lambs were infused with eicosapentaenoic acid (IUGR+EPA; n = 9) or saline (IUGR; n = 8) for 5 days during late gestation and compared to saline-infused controls (n = 11). Circulating eicosapentaenoic acid was 42% less (p 2 but resolved the 67% greater (p p Semitendinosus, longissimus dorsi, and flexor digitorum superficialis muscles were 24–39% lighter (p p p < 0.05) β2 adrenoceptor content in IUGR muscle indicated enhanced inflammatory sensitivity and diminished β2 adrenergic sensitivity. Although IL6R remained elevated, β2 adrenoceptor deficits were resolved in IUGR+EPA muscle, demonstrating a unique underlying mechanism for muscle dysregulation. These findings show that fetal inflammation contributes to IUGR muscle growth deficits and thus may be an effective target for intervention.

Published in Metabolites

ISSN: 2218-1989 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/metabolites

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