Molecular Plant-Microbe Interactions (Jul 2001)

Mutants of Tobacco mosaic virus with Temperature-Sensitive Coat Proteins Induce Heat Shock Response in Tobacco Leaves

  • Harald Jockusch,
  • Christiane Wiegand,
  • Birgit Mersch,
  • Daniela Rajes

DOI
https://doi.org/10.1094/MPMI.2001.14.7.914
Journal volume & issue
Vol. 14, no. 7
pp. 914 – 917

Abstract

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We analyzed, with respect to heat shock proteins (HSPs), systemically reacting tobacco leaves inoculated with Tobacco mosaic virus (TMV), wild-type vulgare, and temperature-sensitive coat protein (CP) mutants Ni 118 (P20L) and flavum (D19A), kept at 23 or 30°C. HSP18 and HSP70 mRNAs and proteins were induced with temperature-sensitive CP mutants after 1 to 2 days at 30°C. After 4 to 6 days, HSP70 was also induced at 23°C. The induction of HSPs paralleled the amount of insoluble TMV CP in leaf extracts, indicating that denatured TMV CP by itself induces a heat-shock response.