Linchuang shenzangbing zazhi (Jan 2016)
Protective effect of Maixuekang on renal function in rats with unilateral ureteral obstruction and its underlying mechanism
Abstract
Objective To observe the protective effect of Maixuekang on renal function in rats with unilateral ureteral obstruction(UUO),and to investigate the possible mechanism.Methods UUO model was established.Rats were divided into three groups:sham operation group,model group and Maixuekang group.ODrresponding intervention was given in each group,and rats of each group were sacrificed at 14 th day after operation.Serum and renal tissue samples were obtained for determination of serum creatinine(SCr),blood urea nitrogen(BUN),cholesterol(TO,triglyceride(TG) levels,observation of the renal tissue pathological changes in different groups,and detection of the expression of tissue type plasminogen activator(t-PA),urokinase type plasminogen activator(u-PA),and plasminogen activator inhibitor-1(PAH) in the renal tissue by immunohistochemistry.Results(1) There was significant difference among the three groups in the morphological changes of kidney.The size and volume of the kidney in sham operation group had no change,and the color was red.The renal volume in model group and Maixuekang group was significantly increased,and renal pelvis and renal calyx on the affected side were expanded.In model group,the color of the kidney become lighter,the kidney cortex became thinner,and the boundary of cortex and medulla were not clear.The renal color in Maixuekang group was slightly red,and the boundary of the renal cortex and medulla was clear.(2) There was no obvious change in renal pathology in sham operation group.Renal tubular injury and renal interstitial fibrosis(RIF) were significantly increased in model group and Maixuekang group as compared with sham operation group(P0.05) in Maixuekang group.Conclusions Maixuekang promoted the degradation of extracellular matrix of kidney by regulating lipid metabolism,down-regulating the expression of PAI-1,up-regulating the expression of t-PA,and protected the RIF and renal function in UUO rats.
