Scientific Reports (May 2024)

O-GlcNAcylation levels remain stable regardless of the anaesthesia in healthy rats

  • Thomas Dupas,
  • Amandine Vergnaud,
  • Thomas Pelé,
  • Angélique Blangy-Letheule,
  • Virginie Aillerie,
  • Martin Bouaud,
  • Angélique Erraud,
  • Anaïs Maillard,
  • Dorian Hassoun,
  • Antoine Persello,
  • Jules Lecomte,
  • Matthieu Rivière,
  • Arnaud Tessier,
  • Aurélia A. Leroux,
  • Bertrand Rozec,
  • Manon Denis,
  • Benjamin Lauzier

DOI
https://doi.org/10.1038/s41598-024-61445-0
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 10

Abstract

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Abstract Anaesthetics are used daily in human and veterinary medicine as well as in scientific research. Anaesthetics have an impact on cell homeostasis especially through modulation of protein post-translational modifications. O-GlcNAcylation, a ubiquitous post-translational modification, plays a role in many biological processes. The aims of this study were to evaluate whether (1) anaesthesia influences O-GlcNAcylation and (2) its stimulation affects physiological parameters. Male Wistar rats (n = 38) were anaesthetized with ketamine-xylazine or isoflurane. They randomly received either an intravenous injection of Ringer's lactate or NButGT (10mg/kg) in order to increase O-GlcNAcylation levels. One hour after induction of anaesthesia, haemodynamic parameters and plasmatic markers were evaluated. Heart, brain and lungs were harvested and O-GlcNAcylation levels and O-GlcNAc-related enzymes were evaluated by western blot. Cardiac and pulmonary O-GlcNAcylation levels and cardiac, cerebral and pulmonary O-GlcNAc associated enzyme expression were not impacted with anaesthesia. Compared with ketamine-xylazine, isoflurane had a lower impact on blood pressure, heart rate and glycaemia. Pharmacological stimulation of O-GlcNAcylation by NButGT did not affect the physiological parameters. This study offers unprecedented insights into the regulation of O-GlcNAcylation and O-GlcNAc related enzymes during anaesthesia. Pharmacological stimulation of O-GlcNAcylation over a 1-h period did not disrupt the physiological balance in healthy anaesthetized rats.

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