Nature Communications (Apr 2019)
Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis
- Tui Neri,
- Emilye Hiriart,
- Patrick P. van Vliet,
- Emilie Faure,
- Russell A. Norris,
- Batoul Farhat,
- Bernd Jagla,
- Julie Lefrancois,
- Yukiko Sugi,
- Thomas Moore-Morris,
- Stéphane Zaffran,
- Randolph S. Faustino,
- Alexander C. Zambon,
- Jean-Pierre Desvignes,
- David Salgado,
- Robert A. Levine,
- Jose Luis de la Pompa,
- André Terzic,
- Sylvia M. Evans,
- Roger Markwald,
- Michel Pucéat
Affiliations
- Tui Neri
- INSERM U-1251, MMG, Aix-Marseille University
- Emilye Hiriart
- INSERM U-1251, MMG, Aix-Marseille University
- Patrick P. van Vliet
- University of California San Diego, Skaggs School of Pharmacy and Pharmaceutical Sciences
- Emilie Faure
- INSERM U-1251, MMG, Aix-Marseille University
- Russell A. Norris
- Department of Anatomy and Cell Biology, Medical University of South Carolina
- Batoul Farhat
- INSERM U-1251, MMG, Aix-Marseille University
- Bernd Jagla
- Institut Pasteur - Cytometry and Biomarkers Unit of Technology and Service, Center for Translational Science and Bioinformatics and Biostatistics Hub – C3BI, USR, 3756 IP CNRS
- Julie Lefrancois
- INSERM U-1251, MMG, Aix-Marseille University
- Yukiko Sugi
- Department of Anatomy and Cell Biology, Medical University of South Carolina
- Thomas Moore-Morris
- INSERM U-1251, MMG, Aix-Marseille University
- Stéphane Zaffran
- INSERM U-1251, MMG, Aix-Marseille University
- Randolph S. Faustino
- Center for Regenerative Medicine, Mayo Clinic
- Alexander C. Zambon
- Department of Biopharmaceutical Sciences, Keck Graduate Institute
- Jean-Pierre Desvignes
- INSERM U-1251, MMG, Aix-Marseille University
- David Salgado
- INSERM U-1251, MMG, Aix-Marseille University
- Robert A. Levine
- Cardiac Ultrasound Laboratory, Harvard Medical School, Massachusetts General Hospital
- Jose Luis de la Pompa
- Intercellular Signaling in Cardiovascular Development & Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC)
- André Terzic
- Center for Regenerative Medicine, Mayo Clinic
- Sylvia M. Evans
- University of California San Diego, Skaggs School of Pharmacy and Pharmaceutical Sciences
- Roger Markwald
- Department of Anatomy and Cell Biology, Medical University of South Carolina
- Michel Pucéat
- INSERM U-1251, MMG, Aix-Marseille University
- DOI
- https://doi.org/10.1038/s41467-019-09459-5
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 14
Abstract
There are few human models that can recapitulate valve development in vitro. Here, the authors derive human pre-valvular endocardial cells (HPVCs) from iPSCs and show they can recapitulate early valvulogenesis, and patient derived HPVCs have features of mitral valve prolapse and identified SHH dysregulation.
