Nature Communications (Feb 2016)

Mito-priming as a method to engineer Bcl-2 addiction

  • Jonathan Lopez,
  • Margaux Bessou,
  • Joel S. Riley,
  • Evangelos Giampazolias,
  • Franziska Todt,
  • Tony Rochegüe,
  • Andrew Oberst,
  • Douglas R. Green,
  • Frank Edlich,
  • Gabriel Ichim,
  • Stephen W. G. Tait

DOI
https://doi.org/10.1038/ncomms10538
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 11

Abstract

Read online

Apoptosis often requires mitochondrial outer membrane permeabilization, a process targeted by Bcl-2-binding BH3 mimetics. Here the authors describe and apply 'mito-priming', a method that allows triggering mitochondrial apoptosis in a synchronous manner, facilitating the investigation of mitochondrial apoptosis and its regulation by Bcl-2 proteins.