Influence of DNA fragmentation in the sperm on the development of embryos in vitro fertilization program

Abstract

The effect of the level of DNA fragmentation in the sperm on the development of early embryos in IVF programs for men with low fertility was investigated. A significant negative association between the level of DNA fragmentation in the sperm and the total blastocyst formation rates and a percent of blastocysts with high quality morphology in patients with impaired fertility is proved (rs = -0,59; rs= -0,56). Evaluation of semen quality in men with low fertility is a necessary step before the use of assisted reproductive technologies. The connection between the levels of sperm DNA fragmentation and the peculiarities of the early development of embryos obtained in programs of extracorporal fertilization has been proved. Assessing the quality of sperm in men with reduced fertility is a necessary measure before using auxiliary reproductive technologies. According to literature, the morphology of the blastocyst does not always depend on chromosomal changes in the embryos. Consequently, it is promising to carry out pre-implantation genetic diagnostics of embryos obtained in programs of extracorporal fertilization in patients with high levels of DNA fragmentation in spermatozoa to study the effect of this violation on the karyotype of embryos.

Keywords

• men fertility
• DNA fragmentation in the sperm
• assisted reproductive technologies