Kinematic and Morphometric Assessment of Fresh Semen, before, during and after Mating Period in Brahman Bulls
Ignacio Araya-Zúñiga,
Francisco Sevilla,
Rafael Molina-Montero,
Eduardo R. S. Roldan,
Manuel Barrientos-Morales,
Miguel A. Silvestre,
Anthony Valverde
Affiliations
Ignacio Araya-Zúñiga
Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica
Francisco Sevilla
Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica
Rafael Molina-Montero
Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica
Eduardo R. S. Roldan
Department of Biodiversity and Evolutionary Biology, National Museum of Natural Sciences, Spanish National Research Council (CSIC), 28006 Madrid, Spain
Manuel Barrientos-Morales
Laboratory of Reproductive Biology, School of Veterinary Medicine and Animal Science, University of Veracruz, Veracruz CP 91710, Mexico
Miguel A. Silvestre
Department of Cellular Biology, Functional Biology and Physical Anthropology, Campus Burjassot, University of Valencia, C/Dr Moliner 50, 46100 Burjassot, Spain
Anthony Valverde
Laboratory of Animal Reproduction, School of Agronomy, Costa Rica Institute of Technology, San Carlos Campus, Alajuela 223-21002, Costa Rica
The objective of the present study was to determine the effects that the reproductive season has on the motility, kinematics, morphology, and sperm morphometry of Brahman bulls evaluated with a commercial CASA system. The experiment was carried out at the Costa Rica Institute of Technology from March to August 2021. A total of eight Brahman bulls were used. A total of 28 ejaculates were collected in the pre-mating period (PMP), during it (DMP), and after it (AMP) using an electroejaculator. The sperm concentration was measured with the Accuread photometer. The motility was measured using a Spermtrack® counting chamber. The analyses were performed with the CASA-Mot ISAS®v1 system. The morphology was analyzed using a microscope with a negative phase contrast objective. Morphometry was evaluated with the CASA-Morph. The sperm concentration did not present differences between the PMP and AMP; however, it was significantly higher than DMP (p > 0.05). Regarding the progressiveness variables, linearity on forward progression (LIN), straightness (STR), and wobble (WOB) were higher (p p p p < 0.05) than AMP. These findings are important to understand the implications of reproductive status on sperm quality and to consider them in andrological evaluations.
