3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy

Luca Pesce; Marina Scardigli; Vladislav Gavryusev; Annunziatina Laurino; Giacomo Mazzamuto; Niamh Brady; Giuseppe Sancataldo; Ludovico Silvestri; Christophe Destrieux; Patrick R. Hof; Irene Costantini; Francesco S. Pavone

doi:10.1038/s42003-022-03390-0

Communications Biology (May 2022)

3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy

Luca Pesce,
Marina Scardigli,
Vladislav Gavryusev,
Annunziatina Laurino,
Giacomo Mazzamuto,
Niamh Brady,
Giuseppe Sancataldo,
Ludovico Silvestri,
Christophe Destrieux,
Patrick R. Hof,
Irene Costantini,
Francesco S. Pavone

Affiliations

Luca Pesce: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence
Marina Scardigli: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence
Vladislav Gavryusev: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence
Annunziatina Laurino: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence
Giacomo Mazzamuto: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence
Niamh Brady: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence
Giuseppe Sancataldo: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence
Ludovico Silvestri: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence
Christophe Destrieux: UMR 1253, iBrain, Université de Tours
Patrick R. Hof: Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai
Irene Costantini: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence
Francesco S. Pavone: European Laboratory for Non-linear Spectroscopy (LENS), University of Florence

DOI: https://doi.org/10.1038/s42003-022-03390-0
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 12

Abstract

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An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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